Effectiveness of an autovaccine in a case of canine papillomavirus in a Great Dane dog in Costa Rica

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DOI:

https://doi.org/10.15381/rivep.v33i1.20187

Keywords:

genotype, sequencing, papillomavirus, pathology, autovaccine, PCR, hyperkeratosis

Abstract

The aim of this study was to determine the effectiveness of an autovaccine for papilloma caused by canine papillomavirus (CPV) in the facial region in a three-month-old Great Dane dog. A group of papillomas were collected from the patient’s face and analyzed using molecular techniques (amplification of a segment of the gene that codes for the L1 protein by PCR and sequencing). For sequence analysis, the BLAST algorithm was used. The histological study was performed by hematoxylin-eosin staining of the tissue and analysis under a 20X light microscope. An autovaccine was developed from the excised papillomas, which was administered intradermally on four occasions at seven-day intervals. The presence of genotype 1 CPV was established in the papilloma analyzed. Histopathology established the formation of exophytic epidermal papillae supported by a fibrovascular chorion with hyperkeratosis (orthokeratotic) and hyperplasia of the stratum spinosum and with keratinocytes (koilocytes). The papillomas disappeared after the third application of the autovaccine. This is the first CPV-1 report in Costa Rica. The autovaccine proved to be an effective treatment in the management of facial papillomatosis in the patient.

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Author Biography

  • Ramsés Alfaro-Mora, Universidad Nacional, Heredia, Costa Rica

    Director de investigación y extensión, Universidad Latina de Costa Rica (2019-presente)
    Profesor de Bioquímica, Ingeniería en Acuicultura, Universidad Tecnica Nacional (2018-presente)
    Coordinador de la Catedra de Química Medicinal, Escuela de Farmacia de ULatina (2015-2019) y UCIMED (2015-2018).
    Profesor de Farmacología para Medicina y Cirugía, Escuela de Medicina ULatina (2013-2019)

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Published

2022-02-25

Issue

Vol. 33 No. 1 (2022)

Section

Comunicaciones

License

Copyright (c) 2022 Ramsés Alfaro-Mora, Gaby Dolz

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Alfaro-Mora, R., & Dolz, G. (2022). Effectiveness of an autovaccine in a case of canine papillomavirus in a Great Dane dog in Costa Rica. Revista De Investigaciones Veterinarias Del Perú, 33(1), e20187. https://doi.org/10.15381/rivep.v33i1.20187