Phenotypic and genotypic characterization of Staphylococcus spp with resistance to methicillin in commercial chickens

Authors

  • Rony Cotaquispe Laboratorio de Biología Molecular, Bioservice SRL, Lima https://orcid.org/0000-0002-8273-4803
  • Ruth Sarmiento Hospital de la Solidaridad, Lima
  • Stephane Lovón Laboratorio de Microbiología, Bioservice SRL, Lima
  • Jorge Rodriguez Universidad Peruana Cayetano Heredia, Lima https://orcid.org/0000-0002-4193-8017

DOI:

https://doi.org/10.15381/rivep.v32i3.20395

Keywords:

methicillin-resistant Staphylococcus aureus, methicillin-resistant coagulase positive staphylococci, resistance genes

Abstract

The study aimed to identify Staphylococcus spp with phenotypes and genotypes of methicillin resistance, as well as to identify variants of the coa + gene polymorphism in S. aureus. In total, 97 strains of Staphylococcus spp isolated from chickens were used and antibiograms and multiplex PCR, conventional PCR and sequencing tests were carried out. The phenotypic results indicate the presence of resistance to 2 MRSA and 26 SCN resistant to methicillin (cefoxitin) and 2 S. aureus and 67 SCN with presumed resistance to methicillin (oxacillin) in the antibiograms. Using PCR, the mecA gene responsible for methicillin resistance was identified in 24 strains: 2 MRSA and 22 SCNRM. In addition, 4 S. aureus genotypes with variants of the coagulase gene polymorphism were identified. The results suggest a constant genetic activity, the appearance of clones and the expression of genes for resistance to the main antibiotics faced by Staphylococcus spp.

Downloads

Download data is not yet available.

Downloads

Published

2021-06-23

Issue

Vol. 32 No. 3 (2021)

Section

Artículos Primarios

License

Copyright (c) 2021 Rony Cotaquispe, Ruth Sarmiento, Stephane Lovón, Jorge Rodriguez

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Cotaquispe, R., Sarmiento, R., Lovón, S., & Rodriguez, J. (2021). Phenotypic and genotypic characterization of Staphylococcus spp with resistance to methicillin in commercial chickens. Revista De Investigaciones Veterinarias Del Perú, 32(3), e20395. https://doi.org/10.15381/rivep.v32i3.20395