Reparative effect of autologous serum in corneal injuries produced by chemical agents. Random double-blind preclinical trial in rabbits

Authors

  • I. Hernández-Patiño Universidad Ricardo Palma, Facultad de Medicina Humana, Instituto de Investigaciones en Ciencias Biomédicas. Lima, Peru https://orcid.org/0000-0002-5654-1194
  • G. Rossani Instituto de InvestigacionUniversidad Ricardo Palma, Facultad de Medicina Humana, Instituto de Investigaciones en Ciencias Biomédicas. Lima, Perues en Ciencias Biomédicas, Facultad de Medicina Humana, Universidad Ricardo Palma https://orcid.org/0000-0002-9405-4821
  • E. Borobio Instituto de Ojos, - Clínica San Pablo, Lima, Peru
  • E. Talavera Universidad Ricardo Palma, Facultad de Medicina Humana, Instituto de Investigaciones en Ciencias Biomédicas. Lima, Peru https://orcid.org/0000-0002-0267-2105
  • M. Pilar Quiñones Hospital Nacional Edgardo Rebagliati Martins, Departamento de Anatomía Patológica. Lima, Peru https://orcid.org/0000-0001-8540-305X
  • R. Rozas LL. Universidad Ricardo Palma, Laboratorio de Anatomía Patológica. Lima, Peru
  • Juan Carlos Roque Universidad Ricardo Palma, Facultad de Medicina Humana, Instituto de Investigaciones en Ciencias Biomédicas. Lima, Peru https://orcid.org/0000-0002-1886-0426
  • M. Jara Universidad Ricardo Palma, Facultad de Ciencias Biológicas, Escuela Profesional de Ciencias Veterinarias. Lima, Peru
  • Jhony A. De la Cruz-Vargas Universidad Ricardo Palma, Facultad de Medicina Humana, Instituto de Investigaciones en Ciencias Biomédicas. Lima, Peru

DOI:

https://doi.org/10.15381/rivep.v32i6.20425

Keywords:

platelet-rich plasma, autologous serum , rabbits, corneal injuries

Abstract

The aim of this study was to determine the reparative effect of autologous serum on corneal lesions of rabbits produced by chemical agents. A preclinical-experimental case-control pilot study was carried out with 10 ocular units obtained from five rabbits. The corneal surfaces were injured by controlled surgical abrasion and subsequent application of 70% alcohol or sulfuric acid pH 1 (battery acid). The cases were treated with autologous serum and the controls with physiological serum. The evolution was followed with fluorescein and photographic recording immediately after the injury occurred, and at 24, 48 and 72 hours. The rabbits were euthanised and the histopathological evaluation of the corneas was made. The cases showed a significant reduction in the perimeter of the lesion and greater transparency (p<0.008). A significant difference was observed in relation to the progression and quality of tissue in the injured area (p<0.05). Histological evaluation showed a more uniform and organized corneal tissue in the cases. It is concluded that autologous serum presented a greater benefit compared to the use of placebo in corneal lesions produced by these chemical agents.

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Published

2021-12-22

Issue

Vol. 32 No. 6 (2021)

Section

Artículos Primarios

License

Copyright (c) 2021 I. Hernández-Patiño, G. Rossani, E. Borobio, E. Talavera, M. Pilar Quiñones, R. Rozas LL., Juan Carlos Roque, M. Jara, Jhony A. De la Cruz-Vargas

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How to Cite

Hernández-Patiño, I., Rossani, G., Borobio, E., Talavera, E., Quiñones, M. P., Rozas LL., R., Roque, J. C., Jara, M., & De la Cruz-Vargas, J. A. (2021). Reparative effect of autologous serum in corneal injuries produced by chemical agents. Random double-blind preclinical trial in rabbits. Revista De Investigaciones Veterinarias Del Perú, 32(6), e20425. https://doi.org/10.15381/rivep.v32i6.20425