Diagnostic comparison between cytological and molecular analysis for the detection of Mycoplasma haemofelis in resident cats of the city of Pereira, Risaralda, Colombia

Authors

  • Lyda Caballero Méndez Universidad Tecnológica de Pereira, Laboratorio Multifuncional. Risaralda, Colombia https://orcid.org/0000-0001-6504-3149
  • Luz Natalia Franco Montoya Universidad Tecnológica de Pereira, Laboratorio Multifuncional. Risaralda, Colombia https://orcid.org/0000-0002-9099-1463
  • Margarita María Mazo Universidad Tecnológica de Pereira, Laboratorio Multifuncional. Risaralda, Colombia https://orcid.org/0000-0003-4928-6903
  • Juan Carlos Sepúlveda Universidad Tecnológica de Pereira, Laboratorio de Biología Molecular. Pereira, Risaralda, Colombia https://orcid.org/0000-0002-5410-5997
  • Esteban Valencia Universidad Tecnológica de Pereira, Laboratorio Multifuncional. Risaralda, Colombia https://orcid.org/0000-0002-5312-8230
  • Tatiana Portilla Universidad Tecnológica de Pereira, Laboratorio Multifuncional. Risaralda, Colombia
  • Leandro Restrepo Bioparque Ukumari, Pereira, Risaralda, Colombia https://orcid.org/0000-0002-3684-5174

DOI:

https://doi.org/10.15381/rivep.v33i1.20432

Keywords:

mycoplasmosis, feline, anaemia, PCR, zoonosis

Abstract

Mycoplasma haemofelis is the most pathogenic haemotropic species that affects felines by parasitizing red blood cells and inducing a haemolytic process that can lead to anaemia. The present study aimed to determine the prevalence of this infection in domestic felines from the city of Pereira, Risaralda, Colombia. Samples from 104 domestic felines between 1 and 10 years of age were analysed. Blood samples were taken for cytological (haematological parameters) and molecular analysis by PCR. Additionally, the possible risk factors associated with the presence of M. haemofelis (age, sex, vaccination status and geographical area of origin) were evaluated. The prevalence by molecular analysis was 80.8%, while it was 80.7% with the Giemsa stain and 81.7% with the Wright stain, and the youngest animals (1-2 years) being the most affected. A significant statistical correlation (p=0.0038) of age with the infection was evidenced as an associated risk factor in this area of the country.

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Published

2022-02-25

Issue

Vol. 33 No. 1 (2022)

Section

Artículos Primarios

License

Copyright (c) 2022 Lyda Caballero Méndez, Luz Natalia Franco Montoya, Margarita María Mazo, Juan Carlos Sepúlveda, Esteban Valencia, Tatiana Portilla, Leandro Restrepo

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

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b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Caballero Méndez, L., Franco Montoya, L. N., Mazo, M. M., Sepúlveda, J. C., Valencia, E., Portilla, T., & Restrepo, L. (2022). Diagnostic comparison between cytological and molecular analysis for the detection of Mycoplasma haemofelis in resident cats of the city of Pereira, Risaralda, Colombia. Revista De Investigaciones Veterinarias Del Perú, 33(1), e20432. https://doi.org/10.15381/rivep.v33i1.20432