Study of the biota of laboratory rats: characterization of commensal Escherichia coli

Authors

  • Xochitl Vega-Manriquez Universidad Autónoma de San Luis Potosí, Facultad de Agronomía y Veterinaria, Mexico https://orcid.org/0000-0003-1672-9413
  • Omar Hernández-Fraga Universidad Autónoma de San Luis Potosí, Facultad de Agronomía y Veterinaria, Mexico
  • Juan Manuel Pinos-Rodríguez Universidad Veracruzana, Facultad de Medicina Veterinaria y Zootecnia, Mexico
  • Cesar Martínez-Benítez Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria (SENASICA), Mexico https://orcid.org/0000-0003-3935-7173
  • Ulises Hernández-Chiñas Universidad Nacional Autónoma de México, Facultad de Medicina, Unidad de Investigación Básico y Clínica en Enfermedades Infecciosas, Mexico
  • Carlos Eslava-Campos Universidad Nacional Autónoma de México, Facultad de Medicina, Unidad de Investigación Básico y Clínica en Enfermedades Infecciosas, Mexico https://orcid.org/0000-0002-6286-6454

DOI:

https://doi.org/10.15381/rivep.v33i2.20868

Keywords:

E. coli, laboratory animals, microbiota

Abstract

The aim if this study was to characterize strains of E. coli isolated from Rattus norvegicus belonging to a level 1 animal facility. Faecal samples were taken from 13 rats. Five colonies were randomly selected from the primary growth of the culture of each sampled individual. Biochemical tests were performed and the pathotype and phylogroup were established in the strains that were identified as E. coli by means of the PCR technique. In addition, tests for biofilm formation and antimicrobial susceptibility were performed. Results showed that 65% (26/40) of the isolated strains corresponded to E. coli and all clustered in phylogroup B2. Six of the E. coli strains were resistant to nitrofurantoin (23%), four were resistant to ampicillin (15%), and three strains were resistant to cefozolin (11%). None showed the formation of biofilms, nor the amplification of genes related to any pathotype. The results denote that E. coli belonging to the biota of laboratory rats presents resistance to antibiotics and the presence of the chuA and yjaA genes associated with the B2 phylogroup strains related to extraintestinal diseases.

Downloads

Download data is not yet available.

Downloads

Published

2022-04-27

Issue

Vol. 33 No. 2 (2022)

Section

Artículos Primarios

License

Copyright (c) 2022 Xochitl Vega-Manriquez, Omar Hernández-Fraga, Juan Manuel Pinos-Rodríguez, Cesar Martínez-Benítez, Ulises Hernández-Chiñas, Carlos Eslava-Campos

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Vega-Manriquez, X., Hernández-Fraga, O., Pinos-Rodríguez, J. M., Martínez-Benítez, C., Hernández-Chiñas, U., & Eslava-Campos, C. (2022). Study of the biota of laboratory rats: characterization of commensal Escherichia coli. Revista De Investigaciones Veterinarias Del Perú, 33(2), e20868. https://doi.org/10.15381/rivep.v33i2.20868