Mentha piperita extract increases survival of Litopenaeus vannamei shrimp experimentally infected with Vibrio spp

Authors

DOI:

https://doi.org/10.15381/rivep.v33i3.21178

Keywords:

shrimp, mortality, alternative treatment, phytobiotic, mint, antibiotic

Abstract

The most important bacterial diseases in Litopenaeus vannamei farming are vibriosis caused by Vibrio spp, which are usually treated with oxytetracycline (OTH). Plant extracts are an alternative for its treatment. The study aimed to determine the effect of three extracts of peppermint (Mentha piperita) on the survival of shrimp experimentally infected with Vibrio spp. Three mint extracts were prepared: ethanolic in a water bath (EBM), in olive oil in a water bath (ABM) and macerated in olive oil (MA). Twenty strains of Vibrio spp were isolated from farmed shrimp and it was performed an in vitro assay for their sensitivity to three doses of the extracts (10, 15 and 20 µl). An in vivo trial was carried out for 16 days with 180 shrimp of 4.5 g infected by immersion with 105 CFU/ml of Vibrio spp strains in three treatments: 1) feed with EBM, 2) with 3‰ OTH and 3) without those additives. The survival rate, growth and load of Vibrio spp in the hepatopancreas were evaluated. It was determined that in vitro, the three types of extracts were able to inhibit Vibrio spp, with 20 µl of EBM being the one with the greatest inhibition, although its power was lower than that of OTH (diameter of the inhibition halo equivalent to 58.2% of that of OTH). In the in vivo assay, EBM showed to be as efficient as OTH in increasing survival rate and growth in weight, as well as in reducing the load of Vibrio spp in the hepatopancreas of infected shrimp (p<0.05). It was concluded that EBM is an alternative as efficient as oxytetracycline to increase the survival of shrimp infected with Vibrio spp.

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Author Biography

  • Alberto Ordinola-Zapata, Universidad Nacional de Tumbes. Tumbes, Perú

    Fisheries Engineer, Master in Aquaculture and Environmental Management. Dr. in Environmental Sciences, with studies completed in the Master in Education and the Master in Molecular Biotechnology. Renacyt Researcher (Register P0013539). Principal professor of undergraduate and postgraduate courses at the Facultad de Ingeniería Pesquera y Ciencias del Mar de la Universidad Nacional de Tumbes, being in charge of different subjects related to the area of scientific research. He has carried out research on shrimp (Litopenaeus vannamei) pathology, as well as several studies on mangrove crab (Ucides occidentalis). He has participated in research in the area of biotechnology, environmental conservation and aquaculture. He has research interests in biotechnology, aquaculture and environmental conservation. He ranked first in academic performance in his undergraduate studies at the Escuela de Ingeniería Pesquera de la Universidad Nacional de Tumbes, and also ranked first in academic performance in the Masters in Aquaculture and Environmental Management at the Universidad Nacional de Tumbes.

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Published

2022-06-29

Issue

Vol. 33 No. 3 (2022)

Section

Artículos Primarios

License

Copyright (c) 2022 Gianmarco Mendoza Noblecilla, Alberto Ordinola-Zapata

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This work is licensed under a Creative Commons Attribution 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

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b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

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How to Cite

Mendoza Noblecilla, G., & Ordinola-Zapata, A. (2022). Mentha piperita extract increases survival of Litopenaeus vannamei shrimp experimentally infected with Vibrio spp. Revista De Investigaciones Veterinarias Del Perú, 33(3), e21178. https://doi.org/10.15381/rivep.v33i3.21178