Evaluation of virulence factors of strains of Salmonella Typhimurium isolated from diseased and healthy guinea pigs (Cavia porcellus)

Authors

  • Carla Duran Gonzalez Unidad de Biología y Genética Molecular, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Peru
  • Luis Luna Espinoza Unidad de Biología y Genética Molecular, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Peru
  • Dennis Carhuaricra Huamán Unidad de Biología y Genética Molecular, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Peru
  • Guillermo Salvatierra Rodríguez Laboratorio de Genómica Microbiana, Universidad Peruana Cayetano Heredia, Lima, Peru
  • Raúl Rosadio Alcántara Unidad de Biología y Genética Molecular, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Peru
  • Lenin Maturrano Hernández Unidad de Biología y Genética Molecular, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Peru https://orcid.org/0000-0001-8819-7335

DOI:

https://doi.org/10.15381/rivep.v32i5.21331

Keywords:

guinea pig, Salmonella Typhimurium, virulence factors, PCR Multiplex

Abstract

The present study evaluated the presence of 10 genes encoding various virulence factors of biological importance in 100 Salmonella Typhimurium isolates, where 90 isolates came from sick guinea pigs and 10 from apparently healthy ones. DNA was extracted from all isolates and analyzed using the Multiple PCR technique to assess the presence of the spvB, spiA, cdtB, sipB, tolC, sitC, lpfC, sifA, sopB and pefA genes. A similar genetic pattern was obtained, with variable detection frequencies greater than 60%, both in healthy and sick guinea pigs, except for the cdtB gene, which was not detected. There were no significant differences between the presence of Salmonella Typhimurium virulence factors isolated from sick guinea pigs and apparently healthy guinea pigs.

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Published

2021-10-27

Issue

Vol. 32 No. 5 (2021)

Section

Artículos Primarios

License

Copyright (c) 2021 Carla Duran Gonzalez, Luis Luna Espinoza, Dennis Carhuaricra Huamán, Guillermo Salvatierra Rodríguez, Raúl Rosadio Alcántara, Lenin Maturrano Hernández

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

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How to Cite

Duran Gonzalez, C., Luna Espinoza, L., Carhuaricra Huamán, D., Salvatierra Rodríguez, G., Rosadio Alcántara, R., & Maturrano Hernández, L. (2021). Evaluation of virulence factors of strains of Salmonella Typhimurium isolated from diseased and healthy guinea pigs (Cavia porcellus). Revista De Investigaciones Veterinarias Del Perú, 32(5), e21331. https://doi.org/10.15381/rivep.v32i5.21331