Effect of Ley-Leygo and Triladyl extenders and two equilibration times on the freezing of boar semen

Authors

  • Erika Abigail Reyes-González Dirección General de Educación Tecnológica Agropecuaria y Ciencias del Mar, Centro de Bachillerato Tecnológico Agropecuario N.13 Xmatkuil, Yucatán, México
  • Fernando Centurión-Castro Universidad Autónoma de Yucatán, Facultad de Medicina Veterinaria y Zootecnia, Departamento de Reproducción Animal y Mejoramiento Genético, Mérida, Yucatán, México
  • Nayeli Uc-Espinosa Dirección General de Educación Tecnológica Agropecuaria y Ciencias del Mar, Centro de Bachillerato Tecnológico Agropecuario N.15 Bolonchén, Campeche, México
  • Jesús Enrique Ek-Mex Dirección General de Educación Tecnológica Agropecuaria y Ciencias del Mar, Centro de Bachillerato Tecnológico Agropecuario N.283 Hocabá, Yucatán, México https://orcid.org/0000-0002-0006-1669
  • Jorge Alonso Peralta-Torres Universidad Juárez Autónoma de Tabasco, División Académica de Ciencias Agropecuarias, México https://orcid.org/0000-0002-8962-6434
  • José Candelario Segura-Correa Universidad Autónoma de Yucatán, Facultad de Medicina Veterinaria y Zootecnia, Departamento de Reproducción Animal y Mejoramiento Genético, Mérida, Yucatán, México https://orcid.org/0000-0003-1329-9948

DOI:

https://doi.org/10.15381/rivep.v33i6.22758

Keywords:

cryopreservation, frozen semen, reproduction, ejaculate

Abstract

The aim of this study was to evaluate the effect of Ley-Leygo and Triladyl® extenders and two equilibrium times on individual motility (IM), plasma membrane integrity (PMI), mitochondrial activity (MA) and intact acrosome (IA) in frozen-thawed boar semen. A rich fraction of a total of 20 ejaculates from five boars of a commercial line was used. All ejaculates had a minimum of 80% motility and 85% normal spermatozoa. Each ejaculate was divided into four aliquots: Ley-Leygo and Triladyl® extenders and two equilibrium times (0 and 1 h). The semen samples were thawed at 37 °C for 20 s. The results of the IM and the PMI did not have significant effects for the factors evaluated (p>0.05). There was a significant difference for MA (p<0.05) between the Ley-Leygo (43.57%) and Triladyl® (51.7%) diluents. The IA was 46% for Ley-Leygo and 25% for Triladyl® (p<0.05). The percentage of IA with the equilibration time of 1 h was greater than that of 0 h (p<0.05). It is concluded that the Triladyl® diluent improved the MA and the equilibration time of 1 h improved the percentage of IA.

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Published

2022-12-22

Issue

Vol. 33 No. 6 (2022)

Section

Artículos Primarios

License

Copyright (c) 2022 Erika Abigail Reyes-González, Fernando Centurión-Castro, Nayeli Uc-Espinosa, Jesús Enrique Ek-Mex, Jorge Alonso Peralta-Torres, José Candelario Segura-Correa

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How to Cite

Reyes-González, E. A., Centurión-Castro, F., Uc-Espinosa, N., Ek-Mex, J. E., Peralta-Torres, J. A., & Segura-Correa, J. C. (2022). Effect of Ley-Leygo and Triladyl extenders and two equilibration times on the freezing of boar semen. Revista De Investigaciones Veterinarias Del Perú, 33(6), e22758. https://doi.org/10.15381/rivep.v33i6.22758