Determination of bacteria and fungi in fresh alpaca semen obtained by three collection techniques

Authors

  • Joel I. Pacheco Curie Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria, Centro de Investigación Estación IVITA - Estación Maranganí. Cusco, Perú https://orcid.org/0000-0001-9978-6522
  • José M. Angulo-Tisoc Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria, Centro de Investigación Estación IVITA - Estación Maranganí. Cusco, Perú https://orcid.org/0000-0003-3238-5462
  • Juan Siuce Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria, Laboratorio de Microbiología y Parasitología Veterinaria. Lima, Perú https://orcid.org/0000-0002-9673-7853
  • Wilber García Vera Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria, Centro de Investigación Estación IVITA - Estación Maranganí. Cusco, Perú https://orcid.org/0000-0002-3666-7267

DOI:

https://doi.org/10.15381/rivep.v33i3.22911

Keywords:

microbiota, semen, contamination, copula

Abstract

The study aimed to describe the microbiota present in alpaca semen. Fifteen semen samples were collected using the artificial vagina, electroejaculation and post-copula techniques (five per technique). In addition, preputial (n=5) and vaginal (n=5) swabs were made. Microbiological cultures were made to determine the bacterial and fungal genera and an assessment of the bacterial load by determining the number of colony-forming units (CFU/ml). Six bacterial genera were identified in the vagina and seven in the prepuce, in addition to a fungal species (Aspergillus fumigatus). Bacteria of the genera Escherichia, Staphylococcus, Streptococcus and Bacillus were the most abundant and present in semen obtained by the three techniques, coinciding with those found in preputial and vaginal swabs. The amount of CFU/ml was similar for the three techniques, while there was no presence of fungi in the semen obtained by electroejaculation, probably due to the previous preputial washing. The presence and quantity of bacterial and fungal genera was higher than that reported in other animal species.

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Published

2022-06-29

Issue

Vol. 33 No. 3 (2022)

Section

Artículos Primarios

License

Copyright (c) 2022 Joel I. Pacheco Curie, José M. Angulo-Tisoc, Juan Siuce, Wilber García Vera

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Pacheco Curie, J. I., Angulo-Tisoc, J. M., Siuce, J., & García Vera, W. (2022). Determination of bacteria and fungi in fresh alpaca semen obtained by three collection techniques. Revista De Investigaciones Veterinarias Del Perú, 33(3), e22911. https://doi.org/10.15381/rivep.v33i3.22911