EVALUATION OF AN IMMUNOHISTOCHEMICAL TECHNIQUE FOR THE DIAGNOSIS OF MIXED MAMMARY TUMORS IN BITCHES

Authors

  • Cyntia Rodríguez C. Laboratorio de Histología, Embriología y Patología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Gilberto Santillán A. Laboratorio de Histología, Embriología y Patología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Rosa Perales C. Laboratorio de Histología, Embriología y Patología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Alfonso Chavera C. Laboratorio de Histología, Embriología y Patología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima

DOI:

https://doi.org/10.15381/rivep.v24i2.2484

Keywords:

mammary neoplasms, immunohistochemistry, cytokeratin, vimentin

Abstract

The aim of the study was to evaluate an immunohistochemical technique for the diagnosis of canine mixed mammary tumors (CMMT) using anti-cytokeratin AE1/AE3 and anti-vimentin monoclonal antibodies that are routinely used in the diagnosis of human mammary tumors. Twenty paraffin tissue blocks of canine mixed mammary tumors, previously diagnosed by hematoxilin-eosin (HE) histopathology were used. Three slides sets, 4 μm thick, were obtained per viability of the tissue, and the other two were used for the immunohistochemistry through the Streptavidin-Biotin-labeled method. Epithelial cells positively reacted against the anti-cytokeratin AE1/AE3 showing a brown colour at the cytoplasma level. Fibroblasts, connective tissue, myoepithelial cells, cartilage cells and bone areas showed a brown colour against the anti-vimentin antibody. All benign tumors reacted positively to both antibodies, whereas 73% malign tumors were positive with the anti-cytokeratin antibody. It is concluded that mixed mammary neoplasm in dogs may be diagnosed by the technique of immunohistochemistry using monoclonal antibodies used in the detection of human mammary tumors. block. One was stained with HE to confirm the

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Published

2013-06-10

Issue

Vol. 24 No. 2 (2013)

Section

Artículos Primarios

License

Copyright (c) 2013 Cyntia Rodríguez C., Gilberto Santillán A., Rosa Perales C., Alfonso Chavera C.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Rodríguez C., C., Santillán A., G., Perales C., R., & Chavera C., A. (2013). EVALUATION OF AN IMMUNOHISTOCHEMICAL TECHNIQUE FOR THE DIAGNOSIS OF MIXED MAMMARY TUMORS IN BITCHES. Revista De Investigaciones Veterinarias Del Perú, 24(2), 176-181. https://doi.org/10.15381/rivep.v24i2.2484