Effect of Insulin-Transferrin-Selenium (ITS) supplementation on development rate and quality of bovine embryos in vitro

Authors

  • Mariano Eliécer Acosta Lobo Universidad Autónoma de las Américas, Facultad de Medicina Veterinaria y Zootecnia, Institución Universitaria Visión de las Américas, Medellín, Colombia https://orcid.org/0000-0002-3043-9319
  • Pablo Andrés Robledo Salgado Centro Internacional de Agricultura Tropical, Palmira, Colombia
  • Luis Fernando Londoño Franco Politécnico Colombiano Jaime Isaza Cadavid, Facultad de Ciencias Agrarias, Medellín, Colombia
  • Viviana Andrea Torres Osorio Universidad CES, Medellín, Colombia
  • Neil A. Vásquez Araque Universidad Nacional de Colombia sede Medellín, Facultad de Ciencias, Escuela de Biociencias, Medellín, Colombia

DOI:

https://doi.org/10.15381/rivep.v35i1.25348

Keywords:

oocytes, embryonic structures, insulin, transferrin, sodium selenite, reactive oxygen species

Abstract

The aim of this study was to evaluate the effect of Insulin-Transferrin-Selenium (ITS) supplementation in embryo culture on the blastocyst rate and embryo quality. The bovine zygotes obtained by in vitro maturation (MIV) and fertilization (IVF) were transferred to Evolve medium in 4 groups (T1: Without ITS supplement; T2: ITS the first 48 h of culture (CIV1): T3: ITS after day 2 of culture (CIV2), T4: ITS during all culture time (CIV1 + CIV2). All procedures were performed at 38.5 °C, 5% CO2 and 90% relative humidity. The cleavage and blastocyst rates were determined at 48 h and day 8 of culture. The total cell number and the production of reactive oxygen species (ROS) in the expanded blastocysts were determined by staining the nucleus with Hoechst and the oxidation of the fluorescent probe dichlorodihydrofluorescein diacetate (H2DCFDA). The T4 cultured embryos presented the highest rate of blastocysts (32.6%) and total cell number (145.6) compared to the control group without STI (26.2% and 98.6, respectively; p<0.05). Likewise, the production of ROS in T4 blastocysts was lower (p<0.05) than that obtained in the other study groups. The results show that the addition of the ITS supplement throughout the embryo culture time has beneficial effects on the percentage of blastocysts and embryo quality, assessed by the number of total cells and the production of ROS.

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Published

2024-02-29

Issue

Vol. 35 No. 1 (2024)

Section

Artículos Primarios

License

Copyright (c) 2024 Mariano Eliécer Acosta Lobo, Pablo Andrés Robledo Salgado, Luis Fernando Londoño Franco, Viviana Andrea Torres Osorio, Neil A. Vásquez Araque

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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How to Cite

Acosta Lobo, M. E., Robledo Salgado, P. A., Londoño Franco, L. F., Torres Osorio, V. A., & Vásquez Araque, N. A. (2024). Effect of Insulin-Transferrin-Selenium (ITS) supplementation on development rate and quality of bovine embryos in vitro. Revista De Investigaciones Veterinarias Del Perú, 35(1), e25348. https://doi.org/10.15381/rivep.v35i1.25348