Identification of antimicrobial peptides from Enterococcus faecium IP5-2a isolated from tilapia gut using mass spectrometry

Authors

  • Arnaldo E. Castañeda Inca´Biotec S.A.C, Tumbes, Peru https://orcid.org/0000-0001-5108-8469
  • Manuel Feria Zevallos Inca´Biotec S.A.C, Tumbes, Peru
  • Adrian E. Zatan Inca´Biotec S.A.C, Tumbes, Peru
  • Odalis E. Toledo Inca´Biotec S.A.C, Tumbes, Peru
  • Jorge L. Aguilar Inca´Biotec S.A.C, Tumbes, Peru
  • Pedro Masías Inca´Biotec S.A.C, Tumbes, Peru
  • Virna Cedeño Inca´Biotec S.A.C, Tumbes, Peru
  • Benoit Diringer Inca´Biotec S.A.C, Tumbes, Peru

DOI:

https://doi.org/10.15381/rivep.v35i1.25580

Keywords:

bioactive peptides, probiotics, lactic acid bacteria, MALDI-TOF/TOF

Abstract

Antimicrobial peptides are small molecules that exhibit strong antagonistic activity against pathogenic microorganisms and are synthesized by a wide variety or organisms, including probiotic bacteria. The aim of the study was to identify peptides with antimicrobial activity in the Enterococcus faecium IP5-2a strain previously isolated from the intestinal tract of Nile tilapia (Oreochromis niloticus). Cellular and extracelullar proteins extraction was performed, followed by 1D SDS-PAGE gel electrophoresis and MALDI-TOF/TOF mass spectrometry. The results revealed peptides with antimicrobial activity, including P54 (SagA) protein, a bacteriocin-like protein and a lantibiotic. These proteins play an important role in the antagonistic capacity of the E. faecium IP52a strain, allowing it to be considered as a potential probiotic in the aquaculture industry.

Downloads

Download data is not yet available.

Downloads

Published

2024-02-29

Issue

Vol. 35 No. 1 (2024)

Section

Artículos Primarios

License

Copyright (c) 2024 Arnaldo E. Castañeda, Manuel Feria Zevallos, Adrian E. Zatan, Odalis E. Toledo, Jorge L. Aguilar, Pedro Masías, Virna Cedeño, Benoit Diringer

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Castañeda, A. E., Feria Zevallos, M., Zatan, A. E., Toledo, O. E., Aguilar, J. L., Masías, P., Cedeño, V., & Diringer, B. (2024). Identification of antimicrobial peptides from Enterococcus faecium IP5-2a isolated from tilapia gut using mass spectrometry. Revista De Investigaciones Veterinarias Del Perú, 35(1), e25580. https://doi.org/10.15381/rivep.v35i1.25580