OPTIMAL TIME FOR RECOVERY AND CRYOPRESERVATION OF EPIDIDYMAL CANINE SPERM AFTER ORCHIECTOMY

Authors

  • Sandra Armas R. Laboratorio de Reproducción Animal, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos. Lima, Perú
  • Víctor Fernández A. Clínica de Animales Menores, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos. Lima, Perú
  • María Vásquez C. Laboratorio de Fisiología Animal, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos. Lima, Perú
  • Alexei Santiani A. Laboratorio de Reproducción Animal, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos. Lima, Perú

DOI:

https://doi.org/10.15381/rivep.v22i3.257

Keywords:

sperm, epididymis, canine, semen cryopreservation

Abstract

The aim of the study was to determine the maximum time to recover and cryopreservespermatozoa from the tail of canine epididymis post orchiectomy. The testes were obtainedthrough orchiectomy from 20 dogs aged 1 to 8 years. The testis were placed in sodiumchloride 0.9% and stored at 5 °C for 0, 24, 48 and 72 hours. Spermatozoa were recoveredby cutting the tail of the epididymis in an extender based on Tris-citrate-fructose. For the cryopreservation process, egg yolk (20%) and glycerol (5%) was added to the dilutedsample (sperm + dilutor). The new dilution was packaged in 0.5 ml straws, which weresubjected to a cooling curve and then placed in liquid nitrogen. Total motility, progressivemotility and functional integrity of the membrane were evaluated. Beforecryopreservation process, total motility after 24 and 72 hours of storage at 5 ºCdecreased (p<0.05). However, total motility found after cryopreservation process variedsignificantly only after 72 hours of storage at 5 ºC (p<0.05). Similar trend was observedfor progressive motility. The functional integrity of membrane, both before and afterthe cryopreservation process, did not suffer significant changes between groups. Theresults showed that it is possible to collect and cryopreserve epididymal sperm until48 hours of orchiectomy.

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Published

2011-09-30

Issue

Vol. 22 No. 3 (2011)

Section

Artículos Primarios

License

Copyright (c) 2011 Sandra Armas R., Víctor Fernández A., María Vásquez C., Alexei Santiani A.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Armas R., S., Fernández A., V., Vásquez C., M., & Santiani A., A. (2011). OPTIMAL TIME FOR RECOVERY AND CRYOPRESERVATION OF EPIDIDYMAL CANINE SPERM AFTER ORCHIECTOMY. Revista De Investigaciones Veterinarias Del Perú, 22(3), 199-205. https://doi.org/10.15381/rivep.v22i3.257