IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF NATIVE STRAINS OF LACTOBACILLUS ISOLATES FROM POULTRY LITTER

Authors

  • Carlos Shiva Laboratorio de Microbiología
  • Luis M. Jara Laboratorio de Biología Molecular, Facultad de Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima, Perú

DOI:

https://doi.org/10.15381/rivep.v24i3.2579

Keywords:

Lactobacillus, broiler, probiotics, isolation, ammonia

Abstract

The aim of this study was to isolate and identify bacteria of Lactobacillus genus from poultry litter with 1, 2 or 3 uses and low ammonia records. Resistance for conditions at pH 5 and 8 was evaluated as well as the ability to inhibit activity in urease bacteria (Staphylococcus spp and Pseudomona spp) isolated from poultry litter. Seventy three suspected colonies of Lactobacillus were identified by culture in selective media and morphological patterns. Gram staining and catalase test revealed that only 19 strains were of Lactobacillus genus. Furthermore, through PCR was found that 53% corresponded to L. reuteri, 32% to L. plantarum, 10% to L. vaginalis and 5% to L. salivarius. All isolates survived at conditions of pH 5 and 8 and all strains produced some type of active biocin against Staphylococci spp or Pseudomona spp which might be useful as additive to regulate the microbiota in poultry litter.

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Published

2013-09-16

Issue

Vol. 24 No. 3 (2013)

Section

Artículos Primarios

License

Copyright (c) 2013 Carlos Shiva, Luis M. Jara

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

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b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

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How to Cite

Shiva, C., & Jara, L. M. (2013). IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF NATIVE STRAINS OF LACTOBACILLUS ISOLATES FROM POULTRY LITTER. Revista De Investigaciones Veterinarias Del Perú, 24(3), 307-315. https://doi.org/10.15381/rivep.v24i3.2579