Detection of Fasciola hepatica by indirect ELISA in sheep and goats from Boavita, Colombia

Authors

  • Sharon E. Cruz- Estupiñan Universidad Pedagógica y Tecnológica de Colombia, Grupo de Investigación en Medicina Veterinaria y Zootecnia – GIDIMEVETZ, Tunja, Colombia https://orcid.org/0000-0003-3480-5627
  • Diana M. Bulla-Castañeda Universidad Pedagógica y Tecnológica de Colombia, Grupo de Investigación en Medicina Veterinaria y Zootecnia – GIDIMEVETZ, Tunja, Colombia https://orcid.org/0000-0002-3740-9454
  • Deisy J. Lancheros-Buitrago Universidad Pedagógica y Tecnológica de Colombia, Grupo de Investigación en Medicina Veterinaria y Zootecnia – GIDIMEVETZ, Tunja, Colombia https://orcid.org/0000-0002-7984-3881
  • Diego J. Garcia-Corredor Universidad Pedagógica y Tecnológica de Colombia, Grupo de Investigación en Medicina Veterinaria y Zootecnia – GIDIMEVETZ, Tunja, Colombia https://orcid.org/0000-0001-5122-5435
  • Julio C. Giraldo Forero Universidad Militar Nueva Granada Bogotá, Facultad de Medicina y Ciencias de la Salud, Grupo de Investigación en Ecoepidemiología y Salud Colectiva, Colombia https://orcid.org/0000-0001-7308-8443
  • Martín O. Pulido-Medellín Universidad Pedagógica y Tecnológica de Colombia, Grupo de Investigación en Medicina Veterinaria y Zootecnia – GIDIMEVETZ, Tunja, Colombia https://orcid.org/0000-0003-4989-1476

DOI:

https://doi.org/10.15381/rivep.v34i5.26378

Keywords:

Fasciola hepatica, sheep, goats, indirect ELISA, seroprevalence

Abstract

The aim of this study was to determine the seroprevalence of Fasciola hepatica by indirect ELISA in sheep and goats from Boavita (Boyaca, Colombia). A descriptive cross-sectional study with simple random sampling was carried out. A total of 297 blood samples from sheep and 337 from goats of different breeds and age groups were collected. The sera were analyzed by the indirect ELISA technique with the commercial ELISA kit BIO K 211 - Monoscreen AbELISA F. hepatica. Additionally, an epidemiological survey was carried out. The seroprevalence of F. hepatica was 67.34% (200/297) in sheep and 59.94% (202/337) in goats. Likewise, seroprevalence was higher in males (sheep: 77.78%, 21/27; goats: 63.89%, 23/36), in sheep older than 3 years (83.33%, 50/60) and in goats less than 1 year of age. (69.77%, 60/86), as well as in Creole breed (sheep: 69.84%; goats: 61.79%). Sheep over 3 years of age were determined as a risk factor, while in goats the Creole breed variables and extensive grazing were identified as risk factors.

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Published

2023-10-31

Issue

Section

Artículos Primarios

How to Cite

Cruz- Estupiñan, S. E., Bulla-Castañeda, D. M., Lancheros-Buitrago, D. J., Garcia-Corredor, D. J., Giraldo Forero, J. C., & Pulido-Medellín, M. O. (2023). Detection of Fasciola hepatica by indirect ELISA in sheep and goats from Boavita, Colombia. Revista De Investigaciones Veterinarias Del Perú, 34(5), e26378. https://doi.org/10.15381/rivep.v34i5.26378