Effect of freezing method and incubation period on post-thawing quality of equine semen

Authors

  • Santiago Caicedo Cadavid Politécnico Colombiano Jaime Isaza Cadavid, Grupo de Investigación en Biotecnología Animal GIBA, Medellín, Colombia https://orcid.org/0000-0003-3969-5666
  • Juan David Montoya Páez Politécnico Colombiano Jaime Isaza Cadavid, Grupo de Investigación en Biotecnología Animal GIBA, Medellín, Colombia https://orcid.org/0000-0003-2272-8185

DOI:

https://doi.org/10.15381/rivep.v36i1.30197

Keywords:

cryopreservation, programmable freezing, nitrogen vapours, freezing protocol, preservation, semen quality

Abstract

Semen cryopreservation produces a high degree of alterations in the sperm that generate a consequent loss of fertility and a lower pregnancy rate when compared to fresh semen. Due to this, the present study aimed to evaluate the effect of the freezing method and the incubation period on the quality of equine semen after thawing. For this, semen was collected from three horses using an artificial vagina. The semen was subjected to two freezing methods: nitrogen vapours and programmable freezer. Once thawed, the semen was kept at 37 °C in a water bath and incubator, and semen quality evaluations were carried out at 0, 1 and 2.5 h. It was determined that the use of nitrogen vapours turns out to be the most suitable for cryopreserving equine semen and that maintaining the thawed semen in an incubator turned out to be much more suitable compared to incubation in a water bath.

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Published

2025-02-28

Issue

Vol. 36 No. 1 (2025)

Section

Artículos Primarios

License

Copyright (c) 2025 Santiago Caicedo Cadavid, Juan David Montoya Páez

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Caicedo Cadavid, S., & Montoya Páez, J. D. (2025). Effect of freezing method and incubation period on post-thawing quality of equine semen. Revista De Investigaciones Veterinarias Del Perú, 36(1), e30197. https://doi.org/10.15381/rivep.v36i1.30197