Antibiotic resistance patterns in bacteria isolated from subclinical mastitis

Authors

  • Katherine Chávez Rosero Escuela Superior Politécnica de Chimborazo, Facultad de Ciencias Pecuarias, Riobamba, Ecuador
  • Luis Condolo Ortiz Escuela Superior Politécnica de Chimborazo, Facultad de Ciencias Pecuarias, Riobamba, Ecuador
  • Pamela Vinueza Veloz Escuela Superior Politécnica de Chimborazo, Facultad de Ciencias Pecuarias, Riobamba, Ecuador
  • Nicole Tiama Paucar Escuela Superior Politécnica de Chimborazo, Facultad de Ciencias Pecuarias, Riobamba, Ecuador

DOI:

https://doi.org/10.15381/rivep.v36i1.30199

Keywords:

antibacterial resistance, bovine mastitis, Ecuador, antibiogram

Abstract

The aim of this study was to assess the resistance of bacteria isolated from subclinical mastitis in crossbred Holstein cows from the Tunshi San Nicolás Community, Chimborazo, Ecuador. Milk from the four mammary quarters of 90 lactating cows was analysed using the California Mastitis Test (CMT). Forty cows had at least one positive quarter. Bacterial culture was performed on Blood Agar and MacConkey Agar and identification was done using Gram staining and biochemical tests. The test for bacterial sensitivity and resistance to antibiotics was performed using the Kirby-Bauer protocol. Gram-positive pathogens were isolated: Staphylococcus spp (84.1%) and Streptococcus spp (15.9%), and Gram-negative pathogens: Escherichia spp (73.8%) and Klebsiella spp (26.2%). Sensitivity to gentamicin was 100% for all isolates, and to tetracycline 80.2%, while resistance was evident to penicillin with 65.4%.

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Published

2025-02-28

Issue

Vol. 36 No. 1 (2025)

Section

Artículos Primarios

License

Copyright (c) 2025 Katherine Chávez Rosero, Luis Condolo Ortiz, Pamela Vinueza Veloz, Nicole Tiama Paucar

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Chávez Rosero, K., Condolo Ortiz, L., Vinueza Veloz, P., & Tiama Paucar, N. (2025). Antibiotic resistance patterns in bacteria isolated from subclinical mastitis. Revista De Investigaciones Veterinarias Del Perú, 36(1), e30199. https://doi.org/10.15381/rivep.v36i1.30199