MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS IN DAIRY CATTLE IN THE LIMA AREA DETECTED THROUGH THREE DIAGNOSTIC TECHNIQUES

Authors

  • José Bustamante V. Facultad de Veterinaria y Zootecnia
  • José Aguilar O. Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima.
  • Martín Ortiz M. Centro de Diagnóstico Sanidad Animal, SENASA, Lima
  • José Bustamante L. Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima

DOI:

https://doi.org/10.15381/rivep.v22i4.339

Keywords:

paratuberculosis, Johne’s disease, Mycobacterium avium subsp, acid-fast bacteria

Abstract

The objective of the study was to determine the presence of Mycobacterium avium subsp. paratuberculosis (Map) in dairy cattle in Lima, Peru. The Intradermal Tuberculin Test was applied to 60 cows from three herds and serum samples were tested using two commercial ELISA kits for antibodies against Map. Twenty-two cows (36.7%) were positive to at least to one of these diagnostic techniques (8 to one of the ELISA tests and 18 to the intradermal test). Feacal samples from the 22 positive animals were cultured on Herrold’s egg-yolk medium (HEYM) yielding five Map positive samples. PCR was used in the isolated strains and Map DNA was confirmed in two cows. A slight concordance (0.15, Kappa test) was found between techniques evaluating cellular and humoral immune response, establishing that these tests are not interchangeable (5.55> 3.84, Mc Nemar test). The results confirm the presence of Map in dairy cattle in Lima, Peru.

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Published

2011-12-30

Issue

Vol. 22 No. 4 (2011)

Section

Artículos Primarios

License

Copyright (c) 2011 José Bustamante V., José Aguilar O., Martín Ortiz M., José Bustamante L.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Bustamante V., J., Aguilar O., J., Ortiz M., M., & Bustamante L., J. (2011). MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS IN DAIRY CATTLE IN THE LIMA AREA DETECTED THROUGH THREE DIAGNOSTIC TECHNIQUES. Revista De Investigaciones Veterinarias Del Perú, 22(4), 394-402. https://doi.org/10.15381/rivep.v22i4.339