GENETIC VARIABILITY OF Gallibacterium anatis STRAINS ISOLATED FROM PERUVIAN COMMERCIAL BIRDS WITH RESPIRATORY INFECTIONS

Authors

  • Karina Mendoza A. Bioservice SRL, Lima
  • Amparo I. Zavaleta Laboratorio de Biología Molecular, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos, Lima.
  • Ysabel Koga Bioservice SRL, Lima
  • Jorge Rodríguez B. Bioservice SRL, Lima
  • Arnaldo Alvarado S. Bioservice SRL, Lima
  • Robert Tinico R. Bioservice SRL, Lima

DOI:

https://doi.org/10.15381/rivep.v25i2.8496

Keywords:

genetic variability, Gallibacterium anatis, commercial poultry, ERIC-PCR, biovar, Peru

Abstract

The purpose of this study was to determine the genetic variability of strains of Gallibacterium anatis isolated in commercial poultry with respiratory symptoms from Arequipa (2), Ica (3), La Libertad (27), Lima (62), Madre de Dios (1) and Ucayali (1), collected from 2007 to 2011. Strains were typified by carbohydrate fermentation tests and sensitivity to nine antimicrobials. The genetic variability of these strains was determined by ERIC-PCR, after identification by PCR using specific primers. The analysis of carbohydrate fermentation profiles corresponded to 10 biovars, all of which were found in Lima and 45.8% of the strains belonged to biovar 4. Concerning antimicrobial susceptibility, 40.8% of strains were resistant to all antimicrobials studied, while 94.8 and 95.8% were resistant to enrofloxacin and ciprofloxacin. By ERIC-PCR 24 DNA profiles associated with biovars were obtained, indicating the existence of intraspecific genetic variability in strains of G. anatis circulating in Peru.

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Published

2014-06-17

Issue

Vol. 25 No. 2 (2014)

Section

Artículos Primarios

License

Copyright (c) 2014 Karina Mendoza A., Amparo I. Zavaleta, Ysabel Koga, Jorge Rodríguez B., Arnaldo Alvarado S., Robert Tinico R.

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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

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b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Mendoza A., K., Zavaleta, A. I., Koga, Y., Rodríguez B., J., Alvarado S., A., & Tinico R., R. (2014). GENETIC VARIABILITY OF Gallibacterium anatis STRAINS ISOLATED FROM PERUVIAN COMMERCIAL BIRDS WITH RESPIRATORY INFECTIONS. Revista De Investigaciones Veterinarias Del Perú, 25(2), 233-244. https://doi.org/10.15381/rivep.v25i2.8496