Sperm viability and acrosome integrity in national frozen bull semen

Authors

  • Próspero Cabrera V. Departamento de Producción Animal, Facultad de Zootecnia, Universidad Nacional Agraria La Molina (UNALM), Lima. Perú
  • César Pantoja A. Programa Doctoral en Ciencia Animal, Facultad de Zootecnia, Universidad Nacional Agraria La Molina (UNALM), Lima

DOI:

https://doi.org/10.15381/rivep.v23i2.899

Keywords:

acrosome integrity, frozen semen, HOST, bulls

Abstract

The deterioration of the sperm membrane and acrosome integrity as a method for predicting fertility in bulls was evaluated. Four bulls (2 Holstein and 2 Brown Swiss) from the National Bank of Semen, Lima-Peru were used. Acrosome integrity, sperm membrane integrity, motility, live sperm cells, volume, and sperm concentration during cooling, freezing and thawing was evaluated in 10 ejaculates per sire. In fresh semen, volume was 4.33 ml; sperm concentration was 922.5 x 106/ml and 78.5% of live cells. The individual progressive motility in diluted semen was 82.7 to 86.0% with significant difference between bulls (p<0.05). Acrosome integrity in chilled semen varied from 59.3 to 69.2% and without statistical difference between bulls. Percentage of sperm with intact acrosome decreased from 65.2 in chilled semen to 48.6% in frozen/thawed semen, without statistical difference between bulls. None significant correlation was found between motility and sperm membrane integrity or acrosome integrity.

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Published

2012-06-29

Issue

Vol. 23 No. 2 (2012)

Section

Artículos Primarios

License

Copyright (c) 2012 Próspero Cabrera V., César Pantoja A.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

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b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Cabrera V., P., & Pantoja A., C. (2012). Sperm viability and acrosome integrity in national frozen bull semen. Revista De Investigaciones Veterinarias Del Perú, 23(2), 192-200. https://doi.org/10.15381/rivep.v23i2.899