Characterization and distribution of potentially avian pathogenic eschericia coli isolates from broilers in Peru

Authors

  • Claudia Carranza Departamento de Dirección Técnica, Innova Andina S.A., Lima, Perú
  • Ricardo León Facultad de Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima, Perú
  • Néstor Falcón Facultad de Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima, Perú
  • Anthony Neumann Danisco, USA
  • Christopher Kromm Danisco, USA

DOI:

https://doi.org/10.15381/rivep.v23i2.901

Keywords:

Escherichia coli, pathogenic, genes, virulence, broilers, Peru

Abstract

Avian colibacillosis produced by Escherichia coli infections cause high mortality and morbidity in poultry farms. The objective of this study was to identify the pathogenic isolates of Peruvian broiler farms based in the presence of five genes encoding the virulence factors: iss, iucC, tsh, cvaC and irp2 by the Multiplex PCR. A total of 36 chicks between 14 and 31 days old from three poultry farms located in the north, centre, and south of Peru were sampled. Three colonies suspected to be E. coli were isolated from the intestine of each bird (n=108 colonies), and were biochemically tested with TSI, SIM, LIA, CITRATE and EMB mediums for the confirmation of the diagnostic. The 41 positive colonies to E. coli were analysed by Multiplex PCR, resulting 65.95% potentially pathogenic (carriers of two or more virulence genes); moreover, 9.8% of the isolates had all five genes. Three pathotypes containing irp2, iucC and tsh genes were found in La Libertad and Lima area.

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Published

2012-06-29

Issue

Vol. 23 No. 2 (2012)

Section

Artículos Primarios

License

Copyright (c) 2012 Claudia Carranza, Ricardo León, Néstor Falcón, Anthony Neumann, Christopher Kromm

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Carranza, C., León, R., Falcón, N., Neumann, A., & Kromm, C. (2012). Characterization and distribution of potentially avian pathogenic eschericia coli isolates from broilers in Peru. Revista De Investigaciones Veterinarias Del Perú, 23(2), 209-219. https://doi.org/10.15381/rivep.v23i2.901