Viral and bacterial coexistence in acute pneumonia in neonatal alpacas
DOI:
https://doi.org/10.15381/rivep.v23i3.914Keywords:
neonatal alpaca, acute pneumonia, PI-3 virus, BRSV, P. multocida, M. haemolyticaAbstract
The etiopathogenesis of acute pneumonia, the second most important cause of mortality among Peruvian neonatal alpacas, is still poorly understood. The objective of this study was to characterize gross and histopathology lesions, as well as to identify viruses [parainfluenza type 3 (PI-3) and bovine respiratory syncytial (BRS)] by direct immunofluorescence test, and isolate bacteria [Pasteurella multocida (PM) and Mannheimia haemolytica (MH)] from 24 fatal acute pneumonia cases of 7-39 days-old neonates. At necropsy the gross lesions corresponded to moderate purulent focal bronchopneumonia or severe necrotic purulent fibrinous (n=13), and moderate to severe pulmonary congestion and edema (n=11). Histopathological analysis revealed: acute, severe, and diffuse necrotizing, fibrinous, suppurative bronchopneumonia (n=3), acute mild to moderate and focally diffuse suppurative bronchopneumonia (n=10), and acute, moderate to severe diffuse congestion and pulmonary edema (n=11). Among these 24 cases, 22 yielded virus identification and/or bacterial isolation. Eight cases were positive to one pathogen (5 for viruses and 3 for bacteria), 10/22 were positive for two pathogens [BRSV plus bacteria (n=7), PI-3 plus bacteria (n=2), and one for both bacteria)], and 4/22 positive for 3 pathogens [BRSV, PI-3 plus bacteria (n=3), and PI-3 plus both bacteria (n=1)]. Among the affected lung tissue, virus was identified 19 times (13 positive for BRSV, 9 for PI-3, and 3/19 for both viruses) whereas bacteria was isolated 14 times [P. multocida (n=8), M. haemolytica (n=6), and both bacteria (n=2)]. The presence of multiple pathogens was observed in 14/22 positive cases with an observation of virus-bacteria association in 13/14 of the cases. The coexistence of BRSV-PM was the most frequently observed (6/13), followed by the simultaneous presence of BRSV-MH (4/13) and PI-3 PM or MH (4/13). The results appear to indicate that acute pneumonia in alpaca neonates may well result from virus and bacterial interactions in a similar way to pneumonic lesions of other ruminants.Downloads
Downloads
Published
Issue
Section
License
Copyright (c) 2012 Erick Cirilo C., Alberto Manchego S., Hermelinda Rivera H., Raúl Rosadio A.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
AUTHORS RETAIN THEIR RIGHTS:
a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.
b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).
c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).