Identification and assessment of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pigs (cavia porcellus)

Authors

  • Juan Anglas P. Laboratorio de Fisiología Animal
  • Sergio Cueva M. Laboratorio de Fisiología Animal
  • María Vásquez C. Laboratorio de Fisiología Animal
  • Boris Lira M. Laboratorio de Fisiología Animal
  • Juan Espinoza B. Laboratorio de Farmacología y Toxicología Veterinaria
  • Juan Lucas L. Laboratorio de Salud Pública y Salud Ambiental, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • José Rodríguez G. Laboratorio de Fisiología Animal

DOI:

https://doi.org/10.15381/rivep.v23i4.932

Keywords:

SGLT1, GLUT2, GLP-1, small intestine, guinea pigs

Abstract

The aim of this study was to identify and assess the presence of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pig (Cavia porcellus). Five animals of 90 days old were used. Samples from the duodenum, jejunum and ileum were collected after slaughtering to carry out immunohistochemistry assays. Expression of SGLT1, GLUT2 and GLP-1 was observed in all three portions of the small intestine, where SGLT1 showed the highest expression, with a strong staining intensity in the apical and medium section of the crypt-villus axis. GLUT2 showed a strong staining intensity throughout the crypt-villus axis in the jejunum. GLP-1 expression was indirectly analyzed by calculating the number of L cells per mm2 of intestinal mucosa, resulting that the jejunum had the largest number (8.46 ±1.16 cells/mm2) (p<0.05). The results suggest that SGLT1 glucose transporter would be the most important in the guinea pig, and unlike other species, GLP-1 may be mostly secreted by the jejunum.

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Published

2012-12-28

Issue

Vol. 23 No. 4 (2012)

Section

Artículos Primarios

License

Copyright (c) 2012 Juan Anglas P., Sergio Cueva M., María Vásquez C., Boris Lira M., Juan Espinoza B., Juan Lucas L., José Rodríguez G.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

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b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

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How to Cite

Anglas P., J., Cueva M., S., Vásquez C., M., Lira M., B., Espinoza B., J., Lucas L., J., & Rodríguez G., J. (2012). Identification and assessment of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pigs (cavia porcellus). Revista De Investigaciones Veterinarias Del Perú, 23(4), 399-405. https://doi.org/10.15381/rivep.v23i4.932