Genetic variability of Plasmodium vivax and patterns of recurrence in asymptomatic malaria at Mazan, Iquitos, Peru

Authors

  • Edward Valencia Ayala Laboratorio de Investigación en enfermedades infecciosas LID, Universidad Peruana Cayetano Heredia.
  • Maritza Calderón Sánchez Laboratorio de Investigación en enfermedades infecciosas LID, Universidad Peruana Cayetano Heredia.
  • Manuel Martín Fasabi Espinar Laboratorio de Investigación en enfermedades infecciosas LID, Universidad Peruana Cayetano Heredia.

DOI:

https://doi.org/10.15381/anales.v73i4.1026

Keywords:

Malaria/vivax, plasmodium vivax, genotype

Abstract

Plasmodium vivax displays a high genetic variability for recurrent episodes of illness. Objectives: To determine the genetic variability of P. vivax and patterns of recurrence in asymptomatic malaria. Design: Descriptive analytical. Setting: Laboratory of Infectious Disease Research, Universidad Peruana Cayetano Heredia. Population: Individuals from Mazan, Iquitos, Peru, a malaria endemic region. Intervention: Between June 2006 and November 2008, 222 individuals were analyzed with two sequential blood samples. Main outcome measures: Identification of P. vivax, genotyping based on gene pvmsp3-α, genetic variability of P. vivax and patterns of recurrence. Results: First evaluation: Positive for P. vivax 191/222 (86%), P. falciparum 2/222 (0.9%), mixed infection 21/222 (9.5%) and negative 8/222 (3.6%). Second evaluation: 180/191 (94.2%) remained positive for P. vivax. Genotyping by nested PCR and enzymatic digestion showed polyclonal haplotypes in 17/180 (9.4%) and monoclonal in 163/180 (90.6%). Different haplotypes (reinfection) were observed in 88/180 (48.9%) and identical haplotypes (relapse) in 75/180 (41.7%). Conclusions: There was P. vivax high genetic variability and patterns of malaria recurrence based on genotyping showed differences between reinfection and relapse in asymptomatic individuals in Mazan, Iquitos.

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Published

2012-12-31

Issue

Vol. 73 No. 4 (2012)

Section

Trabajos originales

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Copyright (c) 2012 Edward Valencia Ayala, Maritza Calderón Sánchez, Manuel Martín Fasabi Espinar

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How to Cite

1.
Valencia Ayala E, Calderón Sánchez M, Fasabi Espinar MM. Genetic variability of Plasmodium vivax and patterns of recurrence in asymptomatic malaria at Mazan, Iquitos, Peru. An Fac med [Internet]. 2012 Dec. 31 [cited 2024 Aug. 16];73(4):285-92. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/1026