Effect of methanol extract of Jatropha macrantha Müll. Arg. in rat erectile dysfunction

Authors

  • Aldo Tinco Universidad Nacional Mayor de San Marcos. Lima, Perú
  • Jorge Arroyo Universidad Nacional de San Cristóbal de Huamanga, Ayacucho, Perú.
  • Pablo Bonilla Universidad Nacional Mayor de San Marcos. Lima, Perú.

DOI:

https://doi.org/10.15381/anales.v72i3.1061

Keywords:

Jatropha macrantha Müll Arg. ‘huanarpo macho’, sexual behavior, nitric oxide, isolated organ, erectile dysfunction.

Abstract

Objectives: To determine the effect of Jatropha macrantha Müll. Arg 'huanarpo macho' methanol extract in rats with induced erectiledysfunction. Design: Experimental. Setting: Pharmacology Laboratory, Faculty of Medicine, Universidad Nacional Mayor de San Marcos,Lima, Peru; Pharmacy Laboratory, Universidad Nacional de San Cristobal de Huamanga, Ayacucho, Peru. Biological material:Plant collected from Ayacucho, wistar male rats 300+/-50 g. Interventions: Plant collection was done in Vilcas Huaman Province - Ayacucho.Sexual behavior, nitric oxide concentration and vasorelaxant effect were determined in isolated rats penis corpus cavernosumdistributed in the following groups: group 1 water 10 mL/kg, group 2 sildenafil 5 mg/kg, groups 3, 4 and 5 respectively methanol extract100, 200 and 300 mg/kg. Organs were isolated in groups receiving 50, 100 and 300 ug/mL of extract, L-arginine 300 uM, 30 um acetylcholine,epinephrine u/mL and sildenafil (3,2 × 10-5 mg/mL). Main outcome measures: Flavonoids isolated, sexual behavior of rats, nitricoxide plasma levels, and cavernous smooth muscle relaxation. Results: By chromatography flavones-like structures were revealed, andby UV spectroscopy and displacement reactions 6-hydroxy-4’,5,7-trimethoxy-flavone, 4’,7-dihydroxy-5,6-dimethoxi-flavone, 7-hydroxy-3’,4’,5’,5,8-pentamethoxyflavone, 4’,7-dihydroxy-3’,5,6-trimethoxy-flavone, and LD50 1357 mg/kg were identified. Sexual behavior wasdose-dependent; oral administration of 300 mg/kg of plant increased frequency of mounts in 75% and raised levels of nitric oxide 85%,while 200 mg/kg dose, mounts were respectively 71.1% and 32.4% (p <0.05). Conclusions: Jatropha macrantha Müll Arg ‘huanarpo macho’methanolic extract had vasorelaxant modulating effect in rats with induced erectile dysfunction with nitric oxide levels increase.

Downloads

Published

2011-09-30

Issue

Vol. 72 No. 3 (2011)

Section

Trabajos originales

License

Copyright (c) 2011 Aldo Tinco, Jorge Arroyo, Pablo Bonilla

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Those authors who have publications with this magazine accept the following terms:

  1. Authors will retain their copyrights and guarantee the journal the right of first publication of their work, which will be simultaneously subject to Creative Commons Attribution License that allows third parties to share the work as long as its author and its first publication this magazine are indicated.
  2. Authors may adopt other non-exclusive licensing agreements for the distribution of the version of the published work (eg, deposit it in an institutional electronic file or publish it in a monographic volume) provided that the initial publication in this magazine is indicated.
  3. Authors are allowed and recommended to disseminate their work over the Internet (eg: in institutional telematic archives or on their website) before and during the submission process, which It can produce interesting exchanges and increase quotes from the published work. (See El efecto del acceso abierto ).

How to Cite

1.
Tinco A, Arroyo J, Bonilla P. Effect of methanol extract of Jatropha macrantha Müll. Arg. in rat erectile dysfunction. An Fac med [Internet]. 2011 Sep. 30 [cited 2024 Jul. 17];72(3):161-8. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/1061