Hypoglycemic effect of Geranium ruizii Hieron. (pasuchaca) ethanolic extract on alloxan-induced hyperglycemia in rats.

Authors

  • Oscar Herrera Calderón Laboratorio de Fisiología y Fisiopatología, Facultad de Farmacia y Bioquímica, Universidad Nacional San Luis Gonzaga, Ica, Perú; Unidad de Posgrado, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos, Lima, Perú
  • Rosa Chinchay Salazar Laboratorio de Fisiología y Fisiopatología, Facultad de Farmacia y Bioquímica, Universidad Nacional San Luis Gonzaga, Ica, Perú;
  • Estela Palomino Ormeño Laboratorio de Fisiología y Fisiopatología, Facultad de Farmacia y Bioquímica, Universidad Nacional San Luis Gonzaga, Ica, Perú;
  • Evelyn Arango Valencia Laboratorio de Fisiología y Fisiopatología, Facultad de Farmacia y Bioquímica, Universidad Nacional San Luis Gonzaga, Ica, Perú;
  • Jorge Arroyo Laboratorio de Farmacología Experimental, Facultad de Medicina Humana, Universidad Nacional Mayor de San Marcos, Lima, Perú; Instituto de Investigaciones Clínicas, Universidad Nacional Mayor de San Marcos, Lima, Perú

DOI:

https://doi.org/10.15381/anales.v76i2.11135

Keywords:

Geranium, hyperglycemia, hypoglycemic effect, TBARS, alloxan, antioxidant.

Abstract

Introduction: Geranium ruizii Hieron. (pasuchaca) is a medicinal plant used by traditional medicine to lower glycemia, in Ancash, Peru. Objective: To determine the hypoglycemic effect of Geranium ruizii ethanolic extract on alloxan-induced hyperglycemia in rats. Design: Experimental. Setting: Laboratorio de Farmacología Experimental, Facultad de Medicina Humana, Universidad San Marcos, Lima, Peru. Biological material: Geranium ruizii, eight weeks female Holtzman rats 200 ± 20 g of body weight. Interventions: Experimental hyperglycemia (>200 mg/dL) was induced with alloxan. Rats were divided into six groups of six rats each. Group I received 2 mL distilled water, groups II, III, IV received respectively Geranium ruizii 50, 150 and 300 mg/kg, group V glibenclamide 5 mg/kg and Group VI insulin 4IU/kg. Main outcome measures: Glycemic level (mg/dL), inhibition of DPPH (%), reduction of reactive species to barbituric acid (TBARS) (nmoles/mL), histopathology study. Results: G. ruizii at 150 mg/kg reduced hyperglycemia 68.58% at 2 h following alloxan induction (Kruskal Wallis; p< 0.001), reduced TBARS in vivo 22.34%, and inhibited DPPH 23.66% to 1.0 ug/mL; the pancreas tissue remained in good condition. Conclusion: The Geranium ruizii (pasuchaca) ethanolic extract showed hypoglycemic effect in alloxan-induced hyperglycemia in rats.

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Published

2015-06-15

Issue

Vol. 76 No. 2 (2015)

Section

Artículo Original

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Copyright (c) 2015 Oscar Herrera Calderón, Rosa Chinchay Salazar, Estela Palomino Ormeño, Evelyn Arango Valencia, Jorge Arroyo

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How to Cite

1.
Herrera Calderón O, Chinchay Salazar R, Palomino Ormeño E, Arango Valencia E, Arroyo J. Hypoglycemic effect of Geranium ruizii Hieron. (pasuchaca) ethanolic extract on alloxan-induced hyperglycemia in rats. An Fac med [Internet]. 2015 Jun. 15 [cited 2024 Jul. 17];76(2):117-22. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/11135