Purification of a 35 kDa protein rich in lysine from Amaranthus caudatus (kiwicha) albumin fraction

Authors

  • Orlinda Villanueva Departamento deMicrobiología y Biotecnología. Facultad de Ciencias, Universidad Nacional del Santa. Chimbote, Perú
  • Inés Arnao Centro de Investigación de Bioquímica y Nutrición. Facultad de Medicina, Universidad Nacional Mayor de San Marcos. Lima, Perú

DOI:

https://doi.org/10.15381/anales.v68i4.1201

Keywords:

Amaranthus, albumins, lysine, amino acids, essential

Abstract

Objective: To purify a protein rich in lysine from Amaranthus caudatus seeds’ albumin fraction. Design: Observational descriptive study. Setting: Biochemistry and Nutrition Research Center, Faculty of Medicine, Universidad Nacional Mayor de San Marcos. Biologic material: Amaranto (Amaranthus caudatus) seeds, Oscar Blanco variety. Interventions: We used both chromatographic (gel filtration) and electrophoretic (SDSPAGE and electroelution) techniques to purify a protein rich in lysine and to determine its molecular weight. The aminoacid composition of the lysine rich protein was obtained by high pressure liquid chromatography (HPLC). Main outcome measures: Isolation and purification of a 35 kDa protein rich in lysine. Results: We isolated a 35 kDa molecular weight protein as determined by SDSPAGE; the essential amino acid composition was similar to the standard protein recommended by the World Health Organization (WHO) with high content of lysine (8,61 moles %). Conclusions: We have isolated and purified a 35 kDa protein rich in lysine from Amaranthus caudatus albumin fraction with an essential amino acid composition similar to that recommended by WHO.

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Published

2007-12-31

Issue

Vol. 68 No. 4 (2007)

Section

Trabajos originales

License

Copyright (c) 2007 Orlinda Villanueva, Inés Arnao

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How to Cite

1.
Villanueva O, Arnao I. Purification of a 35 kDa protein rich in lysine from Amaranthus caudatus (kiwicha) albumin fraction. An Fac med [Internet]. 2007 Dec. 31 [cited 2024 Jul. 2];68(4):344-50. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/1201