Molecular Diagnosis of Mycobacterium tuberculosis in paraffin-embedded pleural biopsies

Authors

  • Helí Barrón Centro de Investigación de Bioquímica y Nutrición Alberto Guzmán Barrón. Facultad de Medicina, UNMSM. Lima, Perú; Hospital San Jose, Minsa. Callao, Perú
  • Mario Monteghirfo Centro de Investigación de Bioquímica y Nutrición Alberto Guzmán Barrón. Facultad de Medicina, UNMSM. Lima, Perú
  • Nelson River Centro de Investigación de Bioquímica y Nutrición Alberto Guzmán Barrón. Facultad de Medicina, UNMSM. Lima, Perú; Morgue Central. Lima, Perú

DOI:

https://doi.org/10.15381/anales.v67i1.1261

Keywords:

Mycobacterium tuberculosis, tuberculosis, pleural, biopsy, diagnosis, clinical

Abstract

Objective: To determine the sensitivity of the test tuberculosis polymerase chain reaction (TB-PCR) compared to fluorochrome auramine “O” staining in histological embedded tissues of pleural biopsy in diagnosis of pleural tuberculosis. Materials and Methods: We used 48 formalin-fixed paraffin-embedded tissue obtained from the archives of the Department of Pathology, Hospital San Jose del Callao, and Instituto Nacional de Enfermedades Neoplasicas. Thirty cases had clinical diagnosis of tuberculosis and 18 different diagnosis. Results: Twenty-nine of 30 cases having tuberculosis were TB-PCR positive. All 18 negative cases were TB-PCR negative. The sensibility and the negative predictive value of TBPCR were 96,7% and 94,7% respectively. On the other hand, the sensitivity and negative predictive value of AFB-Auramine were 58,6% and 56,7%. The positive predictive value was 100% in both cases. Conclusion: TB-PCR is a very sensitive method for TB diagnosis in formalin-fixed, paraffin embedded pleural biopsies.

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Published

2006-03-13

Issue

Vol. 67 No. 1 (2006)

Section

Trabajos originales

License

Copyright (c) 2006 Helí Barrón, Mario Monteghirfo, Nelson River

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How to Cite

1.
Barrón H, Monteghirfo M, River N. Molecular Diagnosis of Mycobacterium tuberculosis in paraffin-embedded pleural biopsies. An Fac med [Internet]. 2006 Mar. 13 [cited 2024 Jul. 17];67(1):11-8. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/1261