Gastroprotective effect and antioxidant capacity of Caesalpinia spinosa ‘tara’ pods aqueous extract in experimental animals

Authors

  • Rosalía Callohuari Egresada de la Maestría en Bioquímica, Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú
  • Miguel Sandoval Vegas Centro de Investigación de Bioquímica y Nutrición, Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú
  • Oscar Huamán Gutiérrez Centro de Investigación de Bioquímica y Nutrición, Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú

DOI:

https://doi.org/10.15381/anales.v78i1.13023

Keywords:

Caesalpinia spinosa, Gastric Mucosa, Gastritis, Mucus Protection, Tara.

Abstract

Objective: To determine the protective effect of the aqueous extract of the pods of Caesalpinia spinosa (Molina) Kuntze “tara” in the gastric mucosa of experimental animals. Design: Experimental. Institution: Research Center on Biochemistry and Nutrition, Faculty of Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru. Biological material: Aqueous extract tara pods (AETP) and 36 albino male rats (217g ± 22 g). Interventions: After a 24-hour fast, the albino rats were divided into 6 groups: GI and GII, NaCl 0.9% to 20 mL/kg; GIII, GIV and GV, AETP at doses of 100, 400 and 800 mg/kg respectively; and GVI, Hidroflat 500 mg/kg. An hour later, gastric injury was caused with 70° ethanol and then gastrectomy was performed. Main outcome measures: Percentage of mucosal protection, using the software ImageJ 1.48v, and the antioxidant activity method 2, 2-diphenyl-1-picrylhydrazyl (DPPH). Results: AETP presented tannins and phenolic compounds in large amounts. High antioxidant activity (IC50 = 1, 1 ± 0.04 μg/mL) was exhibited. The highest percentage of protection was observed at doses of 800 mg/kg (99.7%) and 400 mg/kg (73.1%), p<0, 01, confirmed by histopathological analysis. Conclusions: AETP exhibited antioxidant and protective activity in the experimental model of gastric injury induced by ethanol 70°, in a dose-dependent manner.

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Published

2017-05-16

Issue

Vol. 78 No. 1 (2017)

Section

Artículo Original

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Copyright (c) 2017 Rosalía Callohuari, Miguel Sandoval Vegas, Oscar Huamán Gutiérrez

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How to Cite

1.
Callohuari R, Sandoval Vegas M, Huamán Gutiérrez O. Gastroprotective effect and antioxidant capacity of Caesalpinia spinosa ‘tara’ pods aqueous extract in experimental animals. An Fac med [Internet]. 2017 May 16 [cited 2024 Jul. 17];78(1):61-6. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/13023