Presence of extended-spectrum β-lactamases in two hospitals of Lima, Perú

Authors

  • José Luis Morales Facultad de Farmacia y Bioquímica, UNMSM. Lima, Perú.
  • Karina Reyes Facultad de Farmacia y Bioquímica, UNMSM. Lima, Perú.
  • Mario Monteghirfo Centro de Investigación de Bioquímica y Nutrición. Facultad de Medicina, UNMSM. Lima, Perú.
  • Mirtha Roque Facultad de Farmacia y Bioquímica, UNMSM. Lima, Perú.
  • José Irey Facultad de Farmacia y Bioquímica, UNMSM. Lima, Perú.

DOI:

https://doi.org/10.15381/anales.v66i1.1342

Keywords:

Beta-lactamases, Eescherichia coli, Klebsiella pneumoniae, drug resistance, microbial, multiple.

Abstract

Objective: To study the presence in two hospitals of Lima of extended spectrum β-lactamases (BLEE) and to identify TEM and SHV types produced by Escherichia coli and Klebsiella pneumoniae strains. Material and Methods: The selection and confirmation of BLEE producing strains was done by antimicrobial susceptibility tests. The identification of bla TEM and bla SHV genes was achieved by polymerase chain reaction (PCR) and DNA sequencing. Results: One hundred and thirty seven Escherichia coli and 18 Klebsiella pneumoniae strains were isolated from hospitalized patients between July and September 2000. Most showed high resistance to third-generation cephalosporins and aztreonam;2,9% of all E.coli and 44,4% of all K.pneumoniae isolates were confirmed as BLEE producer strains. All BLEE producer strains were multidrug-resistant and most presented coresistance to trimethoprim/sulfamethoxazole, amikacin, gentamicin and ciprofloxacin. The blaTEM gene was found in 4 strains (3 K. pneumoniae y 1 E. coli) and blaSHV gene in 6 strains (3 K. pneumoniae y 3 E. coli). Sequencing of the corresponding genes confirmed TEM-10 and SHV-5 BLEEs. Conclusion: We determined the presence of TEM and SHV BLEE types, associated to antibiotic multiresistance.

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Published

2005-03-14

Issue

Vol. 66 No. 1 (2005)

Section

Trabajos originales

License

Copyright (c) 2005 José Luis Morales, Karina Reyes, Mario Monteghirfo, Mirtha Roque, José Irey

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How to Cite

1.
Morales JL, Reyes K, Monteghirfo M, Roque M, Irey J. Presence of extended-spectrum β-lactamases in two hospitals of Lima, Perú. An Fac med [Internet]. 2005 Mar. 14 [cited 2024 Jul. 3];66(1):24-32. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/1342