Immunohistochemistry in the diagnosis of yellow fever

Authors

  • ELENA MUÑOZ Centro Nacional de Salud Pública. Instituto Nacional de Salud. Departamento de Patología
  • CECILIA MORÓN Centro Nacional de Salud Pública. Instituto Nacional de Salud. Departamento de Patología
  • ROBERTO KEMPER Hospital Arzobispo Loayza, Departamento de Patología. Lima, Perú
  • RAÚL ROMÁN Centro Nacional de Salud Pública. Instituto Nacional de Salud. Departamento de Patología

DOI:

https://doi.org/10.15381/anales.v65i3.1391

Keywords:

Immunohistochemistry, Yellow fever, Tissues, Antigen.

Abstract

Objective: To determine the presence of yellow fever virus antigen in liver samples. Material and Methods: Immunohistochemistry assay was used in liver samples obtained from 34 patients from 1999 through 2002; they lived in different regions of the country and presented clinical diagnosis of yellow fever. Monoclonal and polyclonal antibodies against yellow fever virus and biotin-streptavidin complex were applied to liver sections (paraffinized-liver). A liver sample from a patient with serological and histophatological diagnosis of yellow fever was used as positive control and a negative control was a liver sample obtained at necropsy from a patient without liver disease. Results: Brown staining of the liver cell cytoplasm was considered positive. The presence of yellow fever virus antigen was confirmed in the 34 liver samples studied from 1999 to 2001. Patients came San Martín, Junín, Cuzco, Loreto, Pasco and Ucayali departments. Conclusion: Immunohistochemistry assay is a useful diagnostic tool for epidemiologic studies due to its high sensitivity and specificity (90%), when a retrospective diagnosis may be performed.

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Published

2004-09-13

Issue

Vol. 65 No. 3 (2004)

Section

Original Breve

License

Copyright (c) 2004 ELENA MUÑOZ, CECILIA MORÓN, ROBERTO KEMPER, RAÚL ROMÁN

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How to Cite

1.
MUÑOZ E, MORÓN C, KEMPER R, ROMÁN R. Immunohistochemistry in the diagnosis of yellow fever. An Fac med [Internet]. 2004 Sep. 13 [cited 2024 Jul. 17];65(3):194-8. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/1391