Biochemical characterization and biological action of an haemorrhagine from the bothrops brazili venom
DOI:
https://doi.org/10.15381/anales.v64i3.1439Keywords:
Bothrops, crotalid venoms, viperidae, immunodiffusion, immunoelectrophoresisAbstract
Objective: To characterize a hemorrhagic protein from Bothrops brazili snake venom and to determine if the polyvalent antibotropic serum is able to neutralize it. Material and Methods: A hemorrhagic protein from Bothrops brazili snake venom was purified through two chromatographical steps: Sephadex G-100 and CM Sephadex C-50, respectively, using 0,05M ammonium acetate buffer pH 7. In the last chromatographical system the protein was eluted after 0,3M sodium chloride was applied; thus, a unique band was achieved by PAGE-SDS. A hemorrhagic action monitored through caseinolytic activity obtained 8,4 folds of purification and the minimum hemorrhagic dose (MHD) was 6,61 ug in albine mice. Results: A structural analysis of associated carbohydrates showed 8,05% of hexoses, 11,62% of hexosamines, and 0,69% of sialic acid; its termostability was detected at 50°C for 10 minutes while total inhibition was observed above this. This protein was very unstable at pH 5, 5mM; EDTA, glutamic acid and glutatión had potent inhibitor effects. In contrast, 10mM of Ca+2 increased the hemorrhagic area. Conclusions: Both inmunodiffusion and inmunoelectrophoresis essays showed that polyvalent botropic antivenom (NIH-Lima) recognized the hemorrhagic protein. Furthermore, in vivo experiments showed that the antivenom was capable to neutralize the whole venom but not purified haemorrhagine.Downloads
Published
2003-09-15
Issue
Section
Trabajos originales
License
Copyright (c) 2003 MARTÍN ISLA, ORESTES MÁLAGA, ARMANDO YARLEQUÉ
![Creative Commons License](http://i.creativecommons.org/l/by-nc-sa/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Those authors who have publications with this magazine accept the following terms:
- Authors will retain their copyrights and guarantee the journal the right of first publication of their work, which will be simultaneously subject to Creative Commons Attribution License that allows third parties to share the work as long as its author and its first publication this magazine are indicated.
- Authors may adopt other non-exclusive licensing agreements for the distribution of the version of the published work (eg, deposit it in an institutional electronic file or publish it in a monographic volume) provided that the initial publication in this magazine is indicated.
- Authors are allowed and recommended to disseminate their work over the Internet (eg: in institutional telematic archives or on their website) before and during the submission process, which It can produce interesting exchanges and increase quotes from the published work. (See El efecto del acceso abierto ).
How to Cite
1.
Isla M, Málaga O, Yarlequé A. Biochemical characterization and biological action of an haemorrhagine from the bothrops brazili venom. An Fac med [Internet]. 2003 Sep. 15 [cited 2024 Jul. 3];64(3):159-66. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/1439