Characterization of the protein pattern and antigen profile of L3 larvae of medical importance in anisakids isolated from commercial fish of the peruvian coast

Authors

  • Rosa Martínez-Rojas Laboratorio de Parasitologia de Fauna Silvestre y Zoonosis, Facultad de Ciencias Biologicas, Universidad Nacional Mayor de San Marcos. Lima, Peru. https://orcid.org/0000-0002-5100-0452
  • Aarón Mondragón-Martínez Laboratorio de Parasitologia de Fauna Silvestre y Zoonosis, Facultad de Ciencias Biologicas, Universidad Nacional Mayor de San Marcos. Lima, Peru. https://orcid.org/0000-0003-0657-0242
  • Eduardo A. Pulido-Murillo Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais. Belo Horizonte, Brasil. https://orcid.org/0000-0002-9039-3068
  • Manuel Tantaleán-Vidaurre Laboratorio de Parasitologia de Fauna Silvestre y Zoonosis, Facultad de Ciencias Biologicas, Universidad Nacional Mayor de San Marcos. Lima, Peru.

DOI:

https://doi.org/10.15381/anales.v81i3.17256

Keywords:

Anisakis, Antigen, Immunoelectrophoresis, Western Blotting, Peru

Abstract

Introduction. Anisakidosis is a zoonosis caused by accidental ingestion of anisakid larvae (L3). Objective. To characterize the protein pattern and antigenic profile of the L3 of Anisakis simplex s.l. (type I), A. physeteris s.l. (type II) and Contracaecum osculatum s.l. commercial fish isolated. Methods. An SDS-PAGE electrophoretic run of the somatic antigens was performed. Rabbits were immunized experimentally and evaluated by EITB. Results. The protein pattern of Anisakis type I showed 12 bands, 18 Anisakis type II and C. osculatum 13, with bands 10 and 35 kDa specific for Anisakis type II, 28 and 77 for C. osculatum, not
present in Anisakis type I. Conclusion. Specific immunogenic bands were determined for Anisakis type I with the molecular weight proteins 11, 14, 25 and 40 kDa, for type II of 9, 10, 12, 35 and 41 kDa and C. osculatum 13, 15, 17, 30 and 47 kDa.

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Published

2020-09-30

Issue

Vol. 81 No. 3 (2020)

Section

Original Breve

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How to Cite

1.
Martínez-Rojas R, Mondragón-Martínez A, Pulido-Murillo EA, Tantaleán-Vidaurre M. Characterization of the protein pattern and antigen profile of L3 larvae of medical importance in anisakids isolated from commercial fish of the peruvian coast. An Fac med [Internet]. 2020 Sep. 30 [cited 2024 Jul. 2];81(3). Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/17256