Molecular characterization and antimicrobial resistance of Vibrio cholerae in Perú, 1991 - 2019
DOI:
https://doi.org/10.15381/anales.v85i2.28433Keywords:
Vibrio cholerae, Microbial Sensitivity Testing, Integrases, Integrons, Microbial drug resistanceAbstract
Introduction. The integrons present in Vibrio cholerae are of interest in public health due to their relationship with multiresistance to antimicrobials. Objetive. Perform a molecular characterization, establish the presence of integrons and evaluate the antimicrobial resistance of Vibrio cholerae strains of clinical and environmental origin isolated in Peru during the years 1991 to 2019. Methods. 34 strains of Vibrio cholerae of clinical and environmental origin isolated during the years 1991 - 2019 were evaluated. All strains were reactivated, then grown in TCBS and in differential media for their taxonomic identification. An antibiogram was performed on all strains according to the disk diffusion method using 16 antimicrobials of clinical use. The genomic DNA was extracted, then the molecular characterization was carried out by PCR and subsequently the amplified products of the integrases were sequenced. Results. All strains evaluated showed 100% sensitivity (n=34) for FEP, CTX, CAZ, CIP and CN. The antimicrobials that exhibited the greatest resistance were AMPR [26.5% (n=9)] and FZR [23.5% (n=8)]. In the present study, 8 antibiotics were established. All strains presented the intl4 gene, except those of environmental origin. All type IV integrases had high homology. Conclusion. Vibrio cholerae presents an increase in antimicrobial resistance. Serogroup O1 had the presence of class IV integrase, as well as high homology.
Downloads
Published
Issue
Section
License
Copyright (c) 2024 Anales de la Facultad de Medicina
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Those authors who have publications with this magazine accept the following terms:
- Authors will retain their copyrights and guarantee the journal the right of first publication of their work, which will be simultaneously subject to Creative Commons Attribution License that allows third parties to share the work as long as its author and its first publication this magazine are indicated.
- Authors may adopt other non-exclusive licensing agreements for the distribution of the version of the published work (eg, deposit it in an institutional electronic file or publish it in a monographic volume) provided that the initial publication in this magazine is indicated.
- Authors are allowed and recommended to disseminate their work over the Internet (eg: in institutional telematic archives or on their website) before and during the submission process, which It can produce interesting exchanges and increase quotes from the published work. (See El efecto del acceso abierto ).
