Evaluation of ELISA antibodies test against P-90 mycobacterial antigen in tuberculosis diagnosis

Authors

  • Alfonzo Uribe Barreto Hospital Nacional Dos de Mayo, Lima, Perú
  • Oswaldo Jave Hospital Nacional Dos de Mayo, Lima, Perú
  • Jaime Alegre Hospital Nacional Dos de Mayo, Lima, Perú
  • Marco Valladares Hospital Nacional Dos de Mayo, Lima, Perú
  • Héctor Díaz Hospital Nacional Dos de Mayo, Lima, Perú
  • Antenor Hernández Hospital Nacional Dos de Mayo, Lima, Perú
  • Antonio Salas Hospital Nacional Dos de Mayo, Lima, Perú
  • Teresa Montoya Hospital Nacional Dos de Mayo, Lima, Perú
  • Elvia Álvarez Hospital Nacional Dos de Mayo, Lima, Perú

DOI:

https://doi.org/10.15381/anales.v62i3.4197

Keywords:

Tuberculosis, pulmonary, serodiagnosis, immunologic techniques, enzime-linked immunosorbent

Abstract

OBJECTIVE: To determine the serologic tuberculosis-specific IgA test’s sensitivity and specificity. DESIGN: Prospective case-control study. SETTING: Hospital Nacional Dos de Mayo from October 1998 through March 1999. MATERIAL AND METHODS: Eighty-one tuberculosis cases diagnosed by smear and culture and/or Koch bacillus positive biopsy, and 86 healthy controls. The tuberculosis serologic diagnosis was done by IgA response to P-90 antigen by immune absorbent enzyme test (EIA-TBÒ, Kreatech; Amsterdam, the Netherlands) prepared from BCG. RESULTS: The test sensitivity was 96,3%, and specificity 77,9%. CONCLUSIONS: IgA detection by Mycobacterium tuberculosis Kp 90 Im CRAC antigen is not useful in tuberculosis diagnosis, especially in the important percentage of BK negative treated patients.
Vol 62, No 3 (2001)

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Published

2001-09-17

Issue

Vol. 62 No. 3 (2001)

Section

Trabajos originales

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Copyright (c) 2001 Alfonzo Uribe Barreto, Oswaldo Jave, Jaime Alegre, Marco Valladares, Héctor Díaz, Antenor Hernández, Antonio Salas, Teresa Montoya, Elvia Álvarez

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How to Cite

1.
Uribe Barreto A, Jave O, Alegre J, Valladares M, Díaz H, Hernández A, et al. Evaluation of ELISA antibodies test against P-90 mycobacterial antigen in tuberculosis diagnosis. An Fac med [Internet]. 2001 Sep. 17 [cited 2024 Jul. 5];62(3):210-4. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/4197