Immunohistochemical Detection of Human Papillomavirus in Cervical Cancer

Authors

  • Eduardo Rengifo Hospital Nacional Arzobispo Loayza Lima, Perú
  • Violeta Seminario Instituto de Patología Facultad de Medicina Universidad Nacional Mayor de San Marcos Lima, Perú
  • Maria Fe Malaspina Centro de Salud Max Arias Scheriber Ministerio de Salud Lima, Perú
  • Augusto Yamada Centro Médico Naval Lima, Perú
  • John Vitorio Instituto de Patología Facultad de Medicina Universidad Nacional Mayor de San Marcos Lima, Perú

DOI:

https://doi.org/10.15381/anales.v61i1.4249

Keywords:

Papillomaviruses, Cervix Neoplasms, Immunohistochemistry, Condylomata Acuminata

Abstract

OBJECTIVES: To establish the prevalence of Human Papillomavirus (HPV) in pre-malignant lesions and invasive cervical lesions, and to assess the sensitivity and specificity of inmunohisto- chemical method to detect HPV antigens with respect to the citologic, colposcopic and anatomopathologic studies. MATERIAL AND METHODS: Cervical biopsy samples were taken under colposcopy among 91 women from Lima, who had a previous abnormal cytologic control. Results of their citologic, colposcopic and biopsy samples, as well as immunohistochemical detection of viral antigens were recorded. RESULTS: HPV was diagnosed 39/91 (43%) by cytologic, 57/91 (63%) by colposcopic, and 78/91 (86%) by histologic methods. HPV antigen expression positivity was 53/91 (56%) by immunohistochemistry. High grade intraepiteliary scamous lesions (ISL) were more frequent than low grade ISL. CONCLUSIONS: Prevalence of HPV among cervical cancer and ISL was high. We considered that immunohistochemical method is not appropriated to HPV infection screenings due to its low sensitivity and specificity.
Vol 61, No 1 (2000)

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Published

2000-03-13

Issue

Vol. 61 No. 1 (2000)

Section

Trabajos originales

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Copyright (c) 2000 Eduardo Rengifo, Violeta Seminario, Maria Fe Malaspina, Augusto Yamada, John Vitorio

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How to Cite

1.
Rengifo E, Seminario V, Malaspina MF, Yamada A, Vitorio J. Immunohistochemical Detection of Human Papillomavirus in Cervical Cancer. An Fac med [Internet]. 2000 Mar. 13 [cited 2024 Jul. 16];61(1):31-6. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/4249