Nitrocellulose Membrane-Based Enzyme-Linked Immunoassay for Dengue Serotype-1 IgM Detection

Authors

  • Segundo León Sandoval Servicio de Medicina Transfusional Centro Médico Naval Lima, Perú

DOI:

https://doi.org/10.15381/anales.v60i2.4475

Keywords:

Dengue, Collodion, Immunoassay, IgM

Abstract

OBJETIVES: To evaluate the sensitivity and specifity of a nitrocellulose membrane-based immunoassay for dengue IgM, with respect to capture enzyme immunoassay, for the diagnosis of dengue virus infection. METHODS: 101 serum samples were processed and divided into 2 groups: 53 from dengue serotype 1 (DEN1) infected patients, and 48 from healthy subjects. Both groups were tested with a nitrocellulose membrane-based IgM capture enzyme immunoassay (NMB-EIA) and also with an ELISA as referential pattern. RESULTS: NMB-EIA testing detected IgM anti-DEN1 in 94,34% of samples from infected patients, and in 14,58% of control samples, whereas ELISA fails to report false positive or false negative results. CONCLUSION: NMB-EIA appears to be a good alternative for dengue infection diagnosis.

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Published

1999-06-14

Issue

Vol. 60 No. 2 (1999)

Section

Trabajos originales

License

Copyright (c) 1999 Segundo León Sandoval

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How to Cite

1.
León Sandoval S. Nitrocellulose Membrane-Based Enzyme-Linked Immunoassay for Dengue Serotype-1 IgM Detection. An Fac med [Internet]. 1999 Jun. 14 [cited 2024 Jul. 17];60(2):110-4. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/4475