Auto Oxidation Kinetics of Ascorbate by Cupric Ions

Authors

  • Luzmila Troncoso Centro de Investigación de Bioquímica y Nutrición, Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú
  • Emilio Guija Centro de Investigación de Bioquímica y Nutrición, Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú

DOI:

https://doi.org/10.15381/anales.v59i2.4632

Keywords:

Ascorbic Acid, Copper, Free Radicals, Redox, Antioxidants

Abstract

Ascorbate autoxidation catalyzed Cu2 + is performed via a first order reaction , a process that is dependent upon the concentration of Cu2 + and that follows a saturation emetic . This suggests that in the sequence an intermediate complex catalyst which would limit the formation of reaction products , one of which is a free hydroxyl radical is formed. Thiourea markedly inhibits autoxidation reaction , while it has no effect glycerol . Ascorbate and Cu2 + , used at concentrations that autoxidation occurs, do not cleave the disulfide bond of DTNB . Histidine and citrate inhibit both autoxidation reaction as breaking of the disulfide bond of DTNB . These results suggest that the rupture of the disulfide bridge of DTNB be realized with the intervention of the hydroxyl free radicals generated by the ascorbate system / Cu + , and in parallel by a process of reduction or hydrolysis .

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Published

1998-06-15

Issue

Vol. 59 No. 2 (1998)

Section

Trabajos originales

License

Copyright (c) 1998 Luzmila Troncoso, Emilio Guija

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How to Cite

1.
Troncoso L, Guija E. Auto Oxidation Kinetics of Ascorbate by Cupric Ions. An Fac med [Internet]. 1998 Jun. 15 [cited 2024 Jul. 8];59(2):105-10. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/4632