Piper aduncum L. (matico) essential oil in vitro antitumoral effect and oral toxicity in mice

Authors

  • Jorge Arroyo Instituto de Investigaciones Clínicas, Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú
  • Oscar Herrera Calderón Laboratorio de Farmacología, Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú
  • Roberto Chávez Asmat Facultad de Farmacia y Bioquímica, Universidad Nacional San Luis Gonzaga, Ica, Perú
  • Edith Ventura Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos, Lima, Perú
  • Jesús Buendía Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos, Lima, Perú
  • José Pacheco Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos, Lima, Perú
  • Robert Palomino Laboratorio de Farmacología, Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú

DOI:

https://doi.org/10.15381/anales.v75i1.6938

Keywords:

Essential oil, cytotoxicity, antitumoral effect, lethal toxicity, Piper aduncum, cell lines

Abstract

Introduction: Piper aduncum (matìco) is a medicinal plant used for gastrointestinal and genitourinary disorders. Objectives: To determine the in vitro antitumoral effect of Piper aduncum (matico) essential oil on seven human tumoral cell lines and its oral toxicity in mice. Design: Experimental. Setting: Faculty of Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru. Biologic material: Human tumoral cell lines HT-29, H-460, MCF-7, M-14, ME-180, DU-145, K-562; and 3T3 fibroblasts and male 40 days post weaning Balb C/53 mice. Interventions: The cell lines HT-29, H-460, MCF-7, M-14, ME-180, DU-145, K-562, and 3T3 were exposed to four different concentrations of Piper aduncum essential oil, and to different 5-fluorouracil concentrations used as a positive control. Cell lines growth inhibition (IC50) was determined using linear regression analysis and DL50 by the number of deaths with each dose. Main outcome measures: Antitumor effect. Results: Piper aduncum essential oil showed cytotoxic activity at IC50 levels > 250 ug/mL on cell lines M-14 (r = -0.99; p < 0.01), DU-145 (r = -0.99; p < 0.01), ME-180 (r = -0.99; p < 0.01). IC50 was between 20 ug/mL and 250 ug/mL on cell lines H-460(r = -0.99; p < 0.01), MCF-7, (r = -0.99; p < 0.01), K562 (r = -0.99; p < 0.01), HT-29 (r = -0.99; p < 0.01). DL50 was > 2 000 mg/kg. Conclusions: P. aduncum essential oil did not show antitumoral effect on seven human tumoral cell lines and it was non toxic.

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Published

2014-03-17

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How to Cite

1.
Arroyo J, Herrera Calderón O, Chávez Asmat R, Ventura E, Buendía J, Pacheco J, et al. Piper aduncum L. (matico) essential oil in vitro antitumoral effect and oral toxicity in mice. An Fac med [Internet]. 2014 Mar. 17 [cited 2024 Jul. 17];75(1):13-8. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/6938