Bidens pilosa chemoprotective effect on induced breast cancer in rats

Authors

  • Jorge Arroyo 1 Instituto de Investigaciones Clínicas, Facultad de Medicina, UNMSM. Lima, Perú. 2 Departamento de Farmacología, Facultad de Medicina, UNMSM. Lima, Perú.
  • Pablo Bonilla Instituto de Ciencias Farmacéuticas y Recursos Naturales, Facultad de Farmacia y Bioquímica, UNMSM. Lima, Perú.
  • Ernesto Ráez Instituto de Patología, Facultad de Medicina, UNMSM. Lima, Perú.
  • Alejandro Barreda Departamento Académico de Ginecología y Obstetricia, Facultad de Medicina, UNMSM. Lima, Perú.
  • Oscar Huamán Centro de Investigación en Bioquímica y Nutrición, Facultad de Medicina, UNMSM. Lima, Perú.

DOI:

https://doi.org/10.15381/anales.v71i3.88

Keywords:

Breast neoplasms, Bidens pilosa, phenolic compounds.

Abstract

Introduction: Bidens pilosa species belonging to the Asteraceae family, known in Peru as love dry bur, is credited with anti-inflammatory, diuretic, hepatoprotective effects. Objectives: To determine the protective effect of phenolic compounds and flavonoids extracted from Bidens pilosa L whole plant on breast cancer induced in rats by 7,12-dimethylbenz (A) anthracene (DMBA). Design: Experimental. Setting: Laboratory of Pharmacology, Faculty of Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru. Biological material: Bidens pilosa L whole plant collected in Santiago de Cao, Ascope, La Libertad, and female rats. Interventions: Phenolic compounds and flavonoids were obtained by rapid column chromatography with solvents of increasing polarity; by thin layer chromatography on a preparative scale four phenolic compounds were isolated and elucidated by UV-Visible spectroscopy with shift reagents. Breast tumors were induced with DMBA administered orally to four groups of rats: normal control group, group with toxic inductor (TI) of cancer, group with TI and ethanolic extract, and group with TI and methanol fraction in doses of 300 mg/kg. Main outcome measures: Induced breast cancer protection. Results: Development of mammary adenocarcinoma tends to slow with treatments, more with the methanol fraction; the oxidative stress marker decreased in the groups treated with the plant, better with the methanol fraction; there were fewer micronuclei (genotoxicity) in animals receiving treatment. Conclusions: Under experimental conditions both Bidens pilosa methanol extracts and fractions halted induced breast cancer progression in rats.

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Published

2010-09-13

Issue

Vol. 71 No. 3 (2010)

Section

Trabajos originales

License

Copyright (c) 2010 Jorge Arroyo, Pablo Bonilla, Ernesto Ráez, Alejandro Barreda, Oscar Huamán

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How to Cite

1.
Arroyo J, Bonilla P, Ráez E, Barreda A, Huamán O. Bidens pilosa chemoprotective effect on induced breast cancer in rats. An Fac med [Internet]. 2010 Sep. 13 [cited 2025 Jun. 13];71(3):153-9. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/88