Copaifera officinalis oil cytoprotector and antisecretory effects in induced gastric lesions in rats

Authors

  • Jorge Arroyo Instituto de Investigaciones Clínicas, Facultad de Medicina, Universidad Nacional Mayor de San Marcos. Lima, Perú. Laboratorio de Farmacología, Facultad de Medicina, Universidad Nacional Mayor de San Marcos. Lima, Perú.
  • Yuan Almora Departamento de Farmacia, Hospital Nacional Hipólito Unanue. Lima, Perú.
  • Mariano Quino Instituto de Investigaciones Clínicas, Facultad de Medicina, Universidad Nacional Mayor de San Marcos. Lima, Perú. Servicio de Gastroenterología, Hospital Nacional Dos de Mayo. Lima, Perú
  • Jaime Martínez Médico Asistente del Seguro Social de Salud (EsSALUD). Lima, Perú.
  • Martín Condorhuamán Médico residente del Hospital Docente Materno Infantil San Bartolomé. Lima, Perú.
  • Marlene Flores Departamento de Anatomía Patológica, Hospital nacional Hipólito Unanue. Lima, Perú.
  • Pablo Bonilla Instituto de Ciencias Farmacéuticas y Recursos Naturales, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos. Lima, Perú.

DOI:

https://doi.org/10.15381/anales.v70i2.955

Keywords:

Stomach ulcer, copaiva, gastrointestinal agents.

Abstract

Objetives: To determine the gastroprotector effect of Copaifera officinalis oil using indomethacin and pyloric ligature in rats. Design: Preclinical study. Setting: Faculties of Medicine, Pharmacy and Biochemistry, National University of San Marcos, Lima, Peru. Biological material: Rats and copaiba oil. Interventions: Copaiba oil was collected in Ucayali, Pucallpa. Cytoprotection was tested with indomethacin considering a normal control group, and indomethacin, copaiba and omeprazole groups. Using visual analogue scale mucosa gastric injuries were referred as those compatible with local necrosis (unviable tissue), hyperemia, flushing, and hemorrhage, and ulcers according to the modified Macallister’s scale. The anti-secretion trial used the pyloric ligature model. Twenty-four albino rats were randomized in three groups: control, copaiba oil 40 mg/kg and omeprazole 10 mg/kg, respectively. After 4 hours of linkage, they were sacrificed. Stomachs were removed, their volume measured carefully and gastric secretion pH determined by potentiometry. Devis’s histopathological evaluation was used. Main outcome measures: Ulcerous injuries. Results: There was 100% cytoprotection with copaiba oil and 97,8% with omeprazole (p<0,0001), ratified by histological findings. Decrease in secretion volume was 79,4% for omeprazole and 42,8% for copaiba oil (p<0,0001) with pH increment. Conclusions: In experimental conditions copaiba oil was effective as gastroprotective agent in gastric ulcers-induced rats.

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Published

2009-06-15

Issue

Vol. 70 No. 2 (2009)

Section

Trabajos originales

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Copyright (c) 2009 Jorge Arroyo, Yuan Almora, Mariano Quino, Jaime Martínez, Martín Condorhuamán, Marlene Flores, Pablo Bonilla

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How to Cite

1.
Arroyo J, Almora Y, Quino M, Martínez J, Condorhuamán M, Flores M, et al. Copaifera officinalis oil cytoprotector and antisecretory effects in induced gastric lesions in rats. An Fac med [Internet]. 2009 Jun. 15 [cited 2024 Jul. 17];70(2):89-96. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/955