Protein and lipid oxidation in guinea pig brain during acute high altitude exposure (4540 m)

Authors

  • Anthony M. Cárdenas Instituto Nacional de Biología Andina, Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú.
  • Luis A. Tataje Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Perú.
  • Edgar A. Florentini Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Perú.
  • Carmen G. Peña Instituto Nacional de Biología Andina, Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú.
  • Elizabeth Carranza A. Instituto Nacional de Biología Andina, Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú.

DOI:

https://doi.org/10.15381/ci.v15i1.3188

Keywords:

Proteins oxidation, lipid peroxidation, antioxidants, altitude, brain

Abstract

The aim of this study was to assess the effect of high altitude exposure time on the oxidation of proteins and lipids from guinea pig brain tissue. Fifty guinea pigs (sea level natives) were taken up to Morococha (4540 m) and sacrificed on days 1, 3, 7 and 14, after arrival. Phospholipase A2 (PLA2) activity, levels of lipid hydroperoxides (LOOH) and malondialdehyde (TBARS) were determined as markers of lipid oxidation. Carbonyl groups (CG) were also determined as markers of protein oxidation. Activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were also determined. PLA2 activity increased until third day. LOOH, TBARS and CG levels were found to be increased the first day; LOOH levels showed a declining trend but TBARS levels remained high, CG concentration decreased below the control group levels on the third day. The activities of antioxidant enzymes GPx and CAT increased from the first day; SOD activity increased until the third day and declined afterwards. It can be concluded that exposure to high altitude directly influences the oxidation of lipids and proteins. The decrease in CG levels might be due to proteolytic system activation, which would degrade the proteins damaged by Reactive Oxygen Species (ROS). The altitude exposure also influences the activity of antioxidant enzymes, especially GPx, which plays an important role in the detoxification of LOOH.

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Published

2012-06-18

Issue

Vol. 15 No. 1 (2012)

Section

Artículos Originales

License

Copyright (c) 2012 Anthony M. Cárdenas, Luis A. Tataje, Edgar A. Florentini, Carmen G. Peña, Elizabeth Carranza A.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

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How to Cite

1.
Cárdenas AM, Tataje LA, Florentini EA, Peña CG, Carranza A. E. Protein and lipid oxidation in guinea pig brain during acute high altitude exposure (4540 m). Ciencia e investigación [Internet]. 2012 Jun. 18 [cited 2024 Aug. 16];15(1):36-41. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/3188