EVALUATION IN VITRO OF THE LEUKOCYTE RESPONSE OF ALPACAS (VICUGNA PACOS)IN PRESENCE OF CLOSTRIDIAL ANTIGENS
DOI:
https://doi.org/10.15381/rivep.v24i4.2742Keywords:
South American camelids, enterotoxemia, cytokines, real time RT-PCRAbstract
The aim of this study was to evaluate the expression of proinflammatory cytokines of alpaca circulating leukocytes when confronting total extract of Clostridum perfringens (structural components and toxins). Whole blood was collected from the jugular vein of 5 female and 5 male adult alpacas. The leukocytes were separated using ammonium chloride to lyse erythrocytes followed by centrifugation and then cultured at a concentration of 500 000 cells/ml of MEM (Minimal Essential Medium) in culture plates of 12 wells. Simultaneously were challenged with total extract of C. perfringens grown in thioglycolate broth at concentrations of 400, 80, 16, 0.8 and 0.2 μg/ml and then quantifying by RT-PCR the expression of cytokines TNF∝, IL-1∝, IL-6 and IL-1ß at 1, 12 and 24 h of exposure. The results showed that low doses of clostridial extracts (0.2 to 0.8 mg/ml) are capable of inducing expression of TNF∝, IL-1∝ and IL-1ß at 1, 12 and 24 h of incubation, whereas higher doses do not exceed the expression of cytokines in unstimulated (control) leukocytes. There was no significant production of IL-6 up to 24 h after exposure to whole extract of C. perfringens. These results indicate that C. perfringens and its toxins at low doses induce activation of circulatory leukocyte in alpacas producing proinflammatory cytokine secretion.Downloads
Downloads
Published
Issue
Section
License
Copyright (c) 2013 Luis Tambillo G., Alberto Manchego S., Kim-Lam Chiok C., Nieves Sandoval C., Juan More B., Hermelinda Rivera G.
![Creative Commons License](http://i.creativecommons.org/l/by-nc-sa/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
AUTHORS RETAIN THEIR RIGHTS:
a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.
b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).
c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).