HYPERTONIC EXTENDERS IN THE CRYOPRESERVATION OF OVINE SEMEN

Authors

  • Hernán Guerrero V. Laboratorio de Salud Pública y Salud Ambiental, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Wilfredo Huanca L. Laboratorio de Salud Pública y Salud Ambiental, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Fernando Raymundo T. Laboratorio de Salud Pública y Salud Ambiental, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Sandra Huerta O. Laboratorio de Salud Pública y Salud Ambiental, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima
  • Daphne Ramos D. Laboratorio de Salud Pública y Salud Ambiental, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima

DOI:

https://doi.org/10.15381/rivep.v20i1.529

Keywords:

cryoprotectant, semen, ram

Abstract

The cryoprotectant effect of two hypertonic extenders (trehalose and lactose) on the post-thawing characteristics of ram semen (n=4) was evaluated. The extender composition included Tris 27.1 g/l, Citric acid 14.0 g/l, Fructose 10.0 g/l, Glycine 10.0 g/l, egg yolk 10.0% (v/v) and Glycerol 6.5% (v/v). Semen was collected in an artificial vagina. Seminal characteristics were: volume: 1.1 ± 0.1 ml, sperm concentration: 3.50 ± 0.1 x 109/ml, individual motility: 87.0 ± 2.4%, wave motility (scale 0-5): 4.4 ± 0.2, live sperms: 90.2 ± 3.8%, and abnormal sperms: 1.8 ± 0.7%. Semen was frozen in 0.5 ml straws and stored in liquid nitrogen. Straws were thawed after 3 months. Results of post-thawing evaluation were: individual motility: 40.3 ± 5.9 and 30.0 ± 5.0%, and live sperms: 34.4 ± 6.6 and 24.3 ± 5.0% for the Trehalose and Lactose extenders respectively. Results showed a better ram semen cryopreservation when the Trehalose extender was used.

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Published

2009-07-15

Issue

Vol. 20 No. 1 (2009)

Section

Artículos Primarios

License

Copyright (c) 2009 Hernán Guerrero V., Wilfredo Huanca L., Fernando Raymundo T., Sandra Huerta O., Daphne Ramos D.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista de Investigaciones Veterinarias del Perú (RIVEP).

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

Guerrero V., H., Huanca L., W., Raymundo T., F., Huerta O., S., & Ramos D., D. (2009). HYPERTONIC EXTENDERS IN THE CRYOPRESERVATION OF OVINE SEMEN. Revista De Investigaciones Veterinarias Del Perú, 20(1), 41-46. https://doi.org/10.15381/rivep.v20i1.529