Coccidiae detection in asymptomatic children by esporulation of fecal samples

Authors

  • ALINA HUIZA Instituto de Medicina Tropical “Daniel A. Carrión”, Facultad de Medicina, UNMSM; Departamento Académico de Microbiología Médica Facultad de Medicina, UNMSM
  • YRMA ESPINOZA Instituto de Medicina Tropical “Daniel A. Carrión”, Facultad de Medicina, UNMSM; Departamento Académico de Microbiología Médica Facultad de Medicina, UNMSM
  • RÓGER ROJAS Facultad de Medicina, UNMSM
  • CARLOS SEVILLA Departamento Académico de Microbiología Médica, Facultad de Medicina, UNMSM
  • PILAR ALVA Departamento Académico de Microbiología Médica, Facultad de Medicina, UNMSM
  • RUBÉN VERÁSTEGUI Hospital Nacional “Daniel A. Carrión”
  • EVA QUISPE Facultad de Medicina, UNMSM
  • GLORIA ROMUALDO Facultad de Medicina, UNMSM
  • ZULEMA ÁNGELES Facultad de Medicina, UNMSM
  • JORGE CANDIOTT Departamento Académico de Microbiología Médica, Facultad de Medicina, UNMSM; Hospital Nacional “Daniel A. Carrión”
  • PEDRO HUAPAYA Instituto de Medicina Tropical “Daniel A. Carrión”, Facultad de Medicina, UNMSM

DOI:

https://doi.org/10.15381/anales.v65i4.1377

Keywords:

Coccidiosis, diagnosis, spores, cryptosporidium, isospora

Abstract

Objective: To estimate intestinal coccidiae infection prevalence of in asymptomatic children from a marginal community in Lima. Material and Methods: Stool samples were collected from 79 asymptomatic children aged 7 months through 7 year-old from Asentamiento Humano “Las Casuarinas de Villa”. Direct smear and colored frotis by Kinyoun technique were prepared. Another portion was placed in small bottles with potasium bicromatum 2,5% to obtain sporulation. After 10 days, frotis were colored by Kinyoun. Results: Direct smear detected one sample with Cryptosporidium (1,3%). In frotis colored before sporulation, 3 with Cryptosporidium (3,8%) were found and one with Isospora belli (1,3%); in frotis colored post-sporulation 6 samples with Cryptosporidium (7.59%) were detected and one with Isospora belli. Conclusion: Sporulation increases Cryptosporidium detection.

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Published

2004-12-31

Issue

Vol. 65 No. 4 (2004)

Section

Trabajos originales

License

Copyright (c) 2004 ALINA HUIZA, YRMA ESPINOZA, RÓGER ROJAS, CARLOS SEVILLA, PILAR ALVA, RUBÉN VERÁSTEGUI, EVA QUISPE, GLORIA ROMUALDO, ZULEMA ÁNGELES, JORGE CANDIOTT, PEDRO HUAPAYA

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How to Cite

1.
HUIZA A, ESPINOZA Y, ROJAS R, SEVILLA C, ALVA P, VERÁSTEGUI R, et al. Coccidiae detection in asymptomatic children by esporulation of fecal samples. An Fac med [Internet]. 2004 Dec. 31 [cited 2024 Jul. 17];65(4):239-42. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/1377