ELISA technique standardization for strongyloidiasis diagnosis

Authors

  • Pedro Huapaya Instituto de Medicina Tropical Daniel Alcides Carrión, UNMSM
  • Yrma Espinoza Instituto de Medicina Tropical Daniel A. Carrión, UNMSM; Departamento Académico de Microbiología Médica, Facultad de Medicina, UNMSM
  • Alina Huiza Instituto de Medicina Tropical Daniel Alcides Carrión, UNMSM; Departamento Académico de Microbiología Médica, Facultad de Medicina, UNMSM
  • Carlos Sevilla Departamento Académico de Microbiología Médica, Facultad de Medicina, UNMSM

DOI:

https://doi.org/10.15381/anales.v63i3.1496

Keywords:

Strongyloides stercoralis, strongyloidiasis, ELISA, serology, diagnosis laboratory.

Abstract

Objective: To standardize ELISA technique for human Strongyloides stercoralis infection diagnosis. Material and methods: A crude antigen was prepared using filariform larvae obtained from positive stool samples cultured with charcoal. Harvested larvae were crushed by sonication and washed by centrifugation in order to obtain protein extracts to be used as antigen. Final protein concentration was 600 µg/mL. Several kinds of ELISA plates were tested and antigen concentration, sera dilution, conjugate dilution and cut off were determined to identify infection. Sera from patients with both hyperinfection syndrome and intestinal infection demonstrated by parasitological examination were positive controls and sera from people living in non-endemic areas with no infection demonstrated by parasitological examination were negative controls. Results: Best values were 5 µg/mL for antigen, 1/64 for sera, 1/1000 for conjugate; optical density values for positive samples were 1,2746 (1,1065 – 1,4206, DS = 0,3284) and for negative samples 0,4457 (0,3324 – 0,5538, DS = 0,2230). Twenty sera samples from positive subjects and one hundred from negative subjects were examined, obtaining 90% sensitivity and 88% specificity. Conclusion: The results show this technique could be useful as strongyloidiasis screening test in population studies.

Downloads

Published

2002-09-16

Issue

Vol. 63 No. 3 (2002)

Section

Trabajos originales

License

Copyright (c) 2002 Pedro huapaya, Yrma Espinoza, Alina Huiza, Carlos Sevilla

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Those authors who have publications with this magazine accept the following terms:

  1. Authors will retain their copyrights and guarantee the journal the right of first publication of their work, which will be simultaneously subject to Creative Commons Attribution License that allows third parties to share the work as long as its author and its first publication this magazine are indicated.
  2. Authors may adopt other non-exclusive licensing agreements for the distribution of the version of the published work (eg, deposit it in an institutional electronic file or publish it in a monographic volume) provided that the initial publication in this magazine is indicated.
  3. Authors are allowed and recommended to disseminate their work over the Internet (eg: in institutional telematic archives or on their website) before and during the submission process, which It can produce interesting exchanges and increase quotes from the published work. (See El efecto del acceso abierto ).

How to Cite

1.
Huapaya P, Espinoza Y, Huiza A, Sevilla C. ELISA technique standardization for strongyloidiasis diagnosis. An Fac med [Internet]. 2002 Sep. 16 [cited 2024 Jul. 27];63(3):179-84. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/1496