Effect of Bixa orellana (achiote) leaves aquous and hydroetanolic extracts on non-enzymatic indicators of paracetamol hepatoxicity in rats

Authors

  • Oscar Huamán Centro de Investigación de Bioquímica y Nutrición.
  • Miguel Sandoval Centro de Investigación de Bioquímica y Nutrición.
  • Elsa Béjar Centro de Investigación de Bioquímica y Nutrición.
  • Zoraida Huamán Facultad de Ciencias Matemática.
  • Víctor Tarazona Facultad de Ciencias Matemática.

DOI:

https://doi.org/10.15381/anales.v74i4.2698

Keywords:

Bixa orellana, TBARS, bilirubin, liver index, hepatoprotective.

Abstract

Objectives: To determine the effect of Bixa orellana leaves aqueous and hydroethanolic extracts on non-enzymatic indicators ofhepatotoxicity in rats subjected to acetaminophen. Design: Experimental. Setting: Research Center of Biochemistry and Nutrition,Universidad Nacional Mayor de San Marcos, Lima, Peru. Biologic material: Thirty-five male rats aged 3 months. Interventions: Rats wererandomized into 5 groups that received orally and for 10 days: positive and negative controls NaCl 0.9%, silymarin 300 mg/kg, aqueousextract 500 mg/kg and hydroethanolic extract 500 mg/kg; after fasting 24 hours, paracetamol 400 mg/kg peroral was administered atthe fifth day, except for the negative control. Under ether anesthesia cardiac puncture was performed to obtain blood. Main outcomemeasures: Liver ratio (liver weight/animal weight x 100), total bilirubin (TB), direct (BD) and indirect (BI) bilirubin, hepatomegaly,thiobarbituric acid reactive species (TBARS) in liver and serum. Results: Treatment with aqueous extract alone decreased the followingindicators: BT and BI (p<0.01), serum TBARS (p<0.05), hepatomegaly -13.2% (p<0.01). The group receiving hydroethanolic extractreduced BT, BI (p<0.01), BD (p<0.05), TBARS in liver (p<0.01), hepatomegaly -9.37%. Conclusions: Bixa orellana (achiote) leavesaqueous and hydroethanolic extracts showed hepatoprotective effect against paracetamol in toxic doses in rats.

Downloads

Published

2013-12-30

Issue

Vol. 74 No. 4 (2013)

Section

Trabajos originales

License

Copyright (c) 2013 Oscar Huamán, Miguel Sandoval, Elsa Béjar, Zoraida Huamán, Víctor Tarazona

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Those authors who have publications with this magazine accept the following terms:

  1. Authors will retain their copyrights and guarantee the journal the right of first publication of their work, which will be simultaneously subject to Creative Commons Attribution License that allows third parties to share the work as long as its author and its first publication this magazine are indicated.
  2. Authors may adopt other non-exclusive licensing agreements for the distribution of the version of the published work (eg, deposit it in an institutional electronic file or publish it in a monographic volume) provided that the initial publication in this magazine is indicated.
  3. Authors are allowed and recommended to disseminate their work over the Internet (eg: in institutional telematic archives or on their website) before and during the submission process, which It can produce interesting exchanges and increase quotes from the published work. (See El efecto del acceso abierto ).

How to Cite

1.
Huamán O, Sandoval M, Béjar E, Huamán Z, Tarazona V. Effect of Bixa orellana (achiote) leaves aquous and hydroetanolic extracts on non-enzymatic indicators of paracetamol hepatoxicity in rats. An Fac med [Internet]. 2013 Dec. 30 [cited 2024 Jul. 17];74(4):279-83. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/2698