Prevalence of E. histolytica and E. dispar by means of microscopy and ELISA in a Suburb of Lima

Authors

  • William Cornejo Medina Departamento Académico de Microbiología Facultad de Medicina Universidad Nacional Mayor de San Marcos Lima, Perú
  • Yrma Espinoza Departamento Académico de Microbiología Facultad de Medicina Universidad Nacional Mayor de San Marcos Lima, Perú
  • Alina Huiza Departamento Académico de Microbiología Facultad de Medicina Universidad Nacional Mayor de San Marcos Lima, Perú
  • Pilar Alva Departamento Académico de Microbiología Facultad de Medicina Universidad Nacional Mayor de San Marcos Lima, Perú
  • Roxana Suárez Departamento Académico de Microbiología Facultad de Medicina Universidad Nacional Mayor de San Marcos Lima, Perú
  • Carlos Sevilla Departamento Académico de Microbiología Facultad de Medicina Universidad Nacional Mayor de San Marcos Lima, Perú
  • César Náquira Departamento Académico de Microbiología Facultad de Medicina Universidad Nacional Mayor de San Marcos Lima, Perú

DOI:

https://doi.org/10.15381/anales.v60i2.4481

Keywords:

Entamoeba histolytica, Enzyme-Linked Immunosorbent Assay, Immunoassay, Microscopy

Abstract

OBJECTIVES: To assess the prevalence of E. histolytica and E. dispar in a marginal population of Callao, Lima, using ELISAs. MATERIAL AND METHODS: Two ELISAs were used, one of them for both Entamoeba species (Entamoeba-ELISA), and an E. histolytica-specific ELISA. RESULTS: 128 stool samples from randomized Callao inhabitants were microscopically examined. Thirteen (10%) were microscopically diagnosed as having E. histolytica or E. dispar infection; and 7 (6%) were positive to Entamoeba–ELISA (sensitivity = 54%). Five of the 115 samples without cysts of E. histolytica or E. dispar were Entamoeba ELISA positive (specificity = 96%). All samples were negative to the E. histolytica-specific ELISA. CONCLUSIONS: The correlation between the microscopical identification and Entamoeba-ELISA was not good, it may be due to an overvaluation of the morphological diagnosis.

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Published

1999-06-14

Issue

Vol. 60 No. 2 (1999)

Section

Original Breve

License

Copyright (c) 1999 William Cornejo Medina, Yrma Espinoza, Alina Huiza, Pilar Alva, Roxana Suárez, Carlos Sevilla, César Náquira

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How to Cite

1.
Cornejo Medina W, Espinoza Y, Huiza A, Alva P, Suárez R, Sevilla C, et al. Prevalence of E. histolytica and E. dispar by means of microscopy and ELISA in a Suburb of Lima. An Fac med [Internet]. 1999 Jun. 14 [cited 2024 Jul. 17];60(2):124-8. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/4481