Inmunohistochemical Identification of Langerhans in Human Cornea

Authors

  • Eduardo Sedano Gelvet Departamento de Ciencias Morfológicas, Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú
  • John Victorio Instituto de Patología, Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú
  • Carlos Neira Instituto de Patología, Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú
  • César Rojas Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú

DOI:

https://doi.org/10.15381/anales.v58i3.4674

Keywords:

Langerhans cells, Human cornea, Immunohistochemistry, Streptavidin-biotin peroxidase, Protein S-100, Muramidase

Abstract

We have achieved the identification of Langerhans cells in human cornea epithelium cross sections, fixed in Zenker’s liquid and embedded in paraffin, according to streptavidin-biotin peroxidase immunohistochemical method, in order to demonstrate protein S-100 and muramidase (lisozime) presence in them.
Langerhans cells were identified easily from the rest of epithelial cells because they were the only elements protein S-100+ and muramidase+, which were stained light brown and showed rounded outlines with one or two prolongations.
Langerhans cells were numerous in the limbic zone and null in the central region.

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Published

1997-09-15

Issue

Vol. 58 No. 3 (1997)

Section

Trabajos originales

License

Copyright (c) 1997 Eduardo Sedano Gelvet, John Victorio, Carlos Neira, César Rojas

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How to Cite

1.
Sedano Gelvet E, Victorio J, Neira C, Rojas C. Inmunohistochemical Identification of Langerhans in Human Cornea. An Fac med [Internet]. 1997 Sep. 15 [cited 2024 Jul. 5];58(3):182-8. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/4674