Ausencia de síntesis de trehalosa en Acidithiobacillus ferrooxidans ATCC 23270 y Acidithiobacillus ferrivorans CF27 a baja temperatura

Autores/as

  • Maria Castañeda-Fernandez Universidad Nacional Mayor de San Marcos, Faculty of Biological Sciences, Laboratory of Molecular Microbiology and Biotechnology, Lima, Peru. https://orcid.org/0000-0003-0163-7460
  • Marcos Sulca Universidad Nacional Mayor de San Marcos, Faculty of Biological Sciences, Laboratory of Molecular Microbiology and Biotechnology, Lima, Peru. https://orcid.org/0000-0001-9056-0068
  • Pablo Ramirez Universidad Nacional Mayor de San Marcos, Faculty of Biological Sciences, Laboratory of Molecular Microbiology and Biotechnology, Lima, Peru. https://orcid.org/0000-0001-9309-7021

DOI:

https://doi.org/10.15381/rpb.v30i1.23034

Palabras clave:

Acidithiobacillus, ensayo enzimático, biosíntesis, trehalosa

Resumen

La trehalosa es un tipo de carbohidrato, que en procariotas protege contra diferentes tipos de estrés y también se utiliza como fuente de almacenamiento de carbono. Hay cuatro formas diferentes de sintetizar trehalosa en Acidithiobacillus ferrivorans y dos en Acidithiobacillus ferrooxidans, pero su propósito sigue siendo desconocido. Este estudio tuvo como objetivo medir la producción de trehalosa en diferentes condiciones mediante su cuantificación en tres medios de cultivo a dos temperaturas diferentes. También se evaluó la cinética de crecimiento de ambas especies y se analizó la concentración de trehalosa durante la fase estacionaria temprana mediante un método enzimático. Los resultados mostraron que el medio 9K modificado con hierro ferroso a 28 °C tuvo la mayor producción de trehalosa, con A. ferrivorans CF27 con una mayor producción de 0.34 µmol/mg de proteína en comparación con A. ferrooxidans ATCC 23270 a 0.31 µmol/mg de proteína. Al utilizar CuS, la producción de trehalosa fue menor, con 0.02 y 0.03 µmol/mg de proteína para A. ferrivorans CF27 y A. ferrooxidans ATCC 23270, respectivamente, mientras que en presencia de zinc no se detectó trehalosa. A 15°C, el método enzimático no detectó trehalosa en los tres medios de cultivo, lo que indicaria que este carbohidrato no protege contra las bajas temperaturas en ninguna de las especies.

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03/14/2023

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Castañeda-Fernandez , Maria, Marcos Sulca, and Pablo Ramirez. 2023. “Ausencia De síntesis De Trehalosa En Acidithiobacillus Ferrooxidans ATCC 23270 Y Acidithiobacillus Ferrivorans CF27 a Baja Temperatura”. Revista Peruana De Biología 30 (1): e23034. https://doi.org/10.15381/rpb.v30i1.23034.